PXD011057-1

PXD011057 is an original dataset announced via ProteomeXchange.

Title	The Leishmania mexicana promastigote flagellar proteome
Description	Leishmania are protists (class: Kinetoplastida) with a single multifunctional flagellum used for motility, attachment to the sand fly vector and sensory functions. To discover the protein composition of the L. mexicana promastigote flagellum, we fractionated L. mexicana promastigotes by mechanical shearing and differential centrifugation into four fractions: detergent soluble (Fs) and insoluble (Fi) fractions of isolated flagella and detergent soluble (Cs) and insoluble fractions (Ci) of deflagellated cell body remnants. A label-free quantitation method (SINQ; Trudgian et al., 2011, Proteomics 10.1002) was used to identify proteins enriched in each of the four fractions. This PRIDE upload contains .RAW, .MGF and .XML files, as well as the SINQ quantification output file “SINQ_raw_data” and the genome sequence annotation used for protein identification “MFiebig_20140619” (Fiebig et al., 2015, PLoS Pathogens II:e1005186). XML files are named SUB7467; MSS10859. .RAW and .MGF files are structured as follows (for each fraction there are eight files, corresponding to individual gel pieces): “Ci: C131121_030; C131121_031; C131121_032; C131121_033; C131121_034; C131121_035; C131121_036; C131121_037”, “Cs: C131121_040; C131121_041; C131121_042; C131121_043; C131121_044; C131121_045; C131121_046; C131121_047”, “Fi: C131121_010; C131121_011; C131121_012; C131121_013; C131121_014; C131121_015; C131121_016; C131121_017”, “Fs: C131121_020; C131121_021; C131121_022; C131121_023; C131121_024; C131121_025; C131121_026; C131121_027”.
HostingRepository	PRIDE
AnnounceDate	2018-12-14
AnnouncementXML	Submission_2018-12-14_03:20:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tom Beneke
SpeciesList	scientific name: Leishmania mexicana MHOM/GT/2001/U1103; NCBI TaxID: 929439;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-11 06:17:24	ID requested
⏵ 1	2018-12-14 03:20:17	announced
2	2019-07-04 04:47:59	announced	Updated publication reference for PubMed record(s): 31242261.

Dataset with its publication pending

submitter keyword: Leishmania, flagellum

Eva Gluenz
contact affiliation	Royal Society University Research Fellow, Group Leader and Research Lecturer at Sir William Dunn School of Pathology (University of Oxford); Fellow by special election at St. Edmund Hall (University of Oxford)
contact email	eva.gluenz@path.ox.ac.uk
lab head
Tom Beneke
contact affiliation	University of Oxford, Sir William Dunn School of Pathology
contact email	tom.beneke@path.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD011057

PRIDE project URI

• PRIDE
  1. PXD011057
     1. Label: PRIDE project
     2. Name: The Leishmania mexicana promastigote flagellar proteome