PXD011050-2

PXD011050 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometric analysis of proteolytic cleavage related to ArtA and PssA from Haloferax volcanii
Description	The S-layer glycoprotein (SLG) of Haloferax volcanii has been shown to be processed and C-terminally linked to a lipid in an archaeosortase A (ArtA)-dependent manner. A C-terminal tripartite structure including a PGF motif is required for the processing of SLG as well as other substrates (Abdul Halim et al., 2013 Mol Microbiol; Abdul Halim et al., 2016 J Bact; Abdul Halim et al., 2017 J Bact; Abdul Halim et al., 2018 Mol Microbiol). In order to gain insights into the mechanism of processing, different cellular fractions (culture supernatant, membrane) from different strains (WT, ArtA KO mutant, ArtA active site mutants, PssA KO mutant) have been analyzed. C-terminal peptides of SLG as well as peptides spanning the PGF motiv were only identified in the mutants lacking processing activity.
HostingRepository	PRIDE
AnnounceDate	2020-05-11
AnnouncementXML	Submission_2020-07-01_22:32:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Schulze
SpeciesList	scientific name: Haloferax volcanii; NCBI TaxID: 2246;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-10 08:27:56	ID requested
1	2020-05-10 22:34:28	announced
⏵ 2	2020-07-01 22:32:44	announced	2020-07-02: Updated publication reference for PubMed record(s): 32561711.

Schulze S, Adams Z, Cerletti M, De Castro R, Ferreira-Cerca S, Fufezan C, Gim, é, nez MI, Hippler M, Jevtic Z, Kn, ü, ppel R, Legerme G, Lenz C, Marchfelder A, Maupin-Furlow J, Paggi RA, Pfeiffer F, Poetsch A, Urlaub H, Pohlschroder M, The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics. Nat Commun, 11(1):3145(2020) [pubmed]

submitter keyword: Haloferax volcanii, Archaeosortase, Lipid anchoring, S-layer glycoprotein

Mechthild Pohlschroder
contact affiliation	Department of Biology, University of Pennsylvania
contact email	pohlschr@sas.upenn.edu
lab head
Stefan Schulze
contact affiliation	University of Pennsylvania
contact email	sschulze@sas.upenn.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011050
     1. Label: PRIDE project
     2. Name: Mass spectrometric analysis of proteolytic cleavage related to ArtA and PssA from Haloferax volcanii