PXD011015-2

PXD011015 is an original dataset announced via ProteomeXchange.

Title	Characterizing N-Glycosylation of Pilins and Flagellins from Haloferax volcanii
Description	Mass spectrometric analysis of cesium chloride purified flagellins and pilins confirmed previously identified N-glycopeptides for FlgA1, revealed additional N-glycopeptides for FlgA1 and FlgA2 and characterized N-glycosylation of PilA1 and PilA2. Analyses were performed using different MS methods (with and withoout applying IS-CID) for samples from wildtype as well as aglB knockout strains.
HostingRepository	PRIDE
AnnounceDate	2020-05-11
AnnouncementXML	Submission_2020-07-01_22:32:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Schulze
SpeciesList	scientific name: Haloferax volcanii (Halobacterium volcanii); NCBI TaxID: 2246;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-05 08:19:54	ID requested
1	2020-05-10 22:33:25	announced
⏵ 2	2020-07-01 22:32:19	announced	2020-07-02: Updated publication reference for PubMed record(s): 32561711, 26966177.

Schulze S, Adams Z, Cerletti M, De Castro R, Ferreira-Cerca S, Fufezan C, Gim, é, nez MI, Hippler M, Jevtic Z, Kn, ü, ppel R, Legerme G, Lenz C, Marchfelder A, Maupin-Furlow J, Paggi RA, Pfeiffer F, Poetsch A, Urlaub H, Pohlschroder M, The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics. Nat Commun, 11(1):3145(2020) [pubmed]

Esquivel RN, Schulze S, Xu R, Hippler M, Pohlschroder M, Identification of Haloferax volcanii Pilin N-Glycans with Diverse Roles in Pilus Biosynthesis, Adhesion, and Microcolony Formation. J Biol Chem, 291(20):10602-14(2016) [pubmed]

curator keyword: Biological

submitter keyword: Haloferax volcanii, N-glycosylation, Archaea, Type IV pili, Flagella

Mechthild Pohlschroder
contact affiliation	Department of Biology, University of Pennsylvania
contact email	pohlschr@sas.upenn.edu
lab head
Stefan Schulze
contact affiliation	University of Pennsylvania
contact email	sschulze@sas.upenn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD011015

PRIDE project URI

• PRIDE
  1. PXD011015
     1. Label: PRIDE project
     2. Name: Characterizing N-Glycosylation of Pilins and Flagellins from Haloferax volcanii