PXD011013 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tunneling nanotubes contribute to the stroma-mediated imatinib resistance of leukemic cells |
Description | Intercellular communication within the bone marrow niche significantly influences leukemogenesis and the sensitivity of leukemic cells to therapy. However, the landscape of possible cell-cell interactions is still incomplete. Tunneling nanotubes (TNTs) are a novel mode of intercellular cross-talk. They are long, thin membranous conduits that enable the direct transfer of various cargo between cells. The present study found that TNTs are formed between leukemic and bone marrow stromal cells. Confocal three-dimensional reconstructions, correlative light-electron microscopy, and electron tomography provided evidence that TNTs transfer cellular vesicles between cells. The quantitative analysis demonstrated the stimulation of TNT-mediated vesicle transfer from stromal cells to leukemic cells by the stromal component. The vesicular cargo that was received from stroma cells conferred resistance to anti-leukemic treatment. Moreover, specific sets of proteins with a potential role in survival and the drug response were transferred within these vesicles. Altogether, we found that TNTs are involved in a novel and potent mechanism that participates in leukemia-stroma cross-talk and the stroma-mediated cytoprotection of leukemic cells. Our findings implicate TNT connections as a possible target for therapeutic interventions within the leukemia microenvironment to attenuate stroma-conferred protection. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-16 |
AnnouncementXML | Submission_2022-02-16_05:33:48.250.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dominik Cysewski |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-09-05 06:51:01 | ID requested | |
⏵ 1 | 2022-02-16 05:33:48 | announced | |
2 | 2023-11-14 08:49:36 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
curator keyword: Biomedical |
submitter keyword: human, nanotubues, leukemic cells, silac |
Contact List
Katarzyna Piwocka |
contact affiliation | 1Laboratory of Cytometry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland |
contact email | k.piwocka@nencki.gov.pl |
lab head | |
Dominik Cysewski |
contact affiliation | Institute of Biochemistry and Biophysics Polish Academy of Sciences |
contact email | dominikcysewski@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011013
- Label: PRIDE project
- Name: Tunneling nanotubes contribute to the stroma-mediated imatinib resistance of leukemic cells