PXD010962 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Naja annulifera Snake: New Insights into the Venom Components and Pathogenesis of Envenomation |
Description | Naja annulifera is a dangerous snake that belongs to the Elapidae family. It is found in some of the countries in Sub-Saharan Africa and has caused several accidents in humans and dogs. In this study, we characterized some of the biochemical, toxic and immunogenic properties of N. annulifera venom. We showed that the venom is composed of several proteins, some of which display enzymatic activities, such as phospholipase A2, hyaluronidase, metalloproteinases and serine proteinases. The venom promoted disturbances in the human coagulation system and was cytotoxic to human epidermal cells. Using a mouse model, we showed that the venom promotes local reactions that were reduced with anti-inflammatory drugs. The venom caused systemic inflammation, lung hemorrhage and death. Further, the venom stimulated production of high antibody titers when injected into mice and the antiserum produced was able to inhibit venom-induced death. We therefore conclude that N. annulifera venom contains toxins that play a role in the toxic and inflammatory effects that contribute to the clinical manifestations and death. Moreover, the venom is immunogenic, an important aspect for the production an efficient N. annulifera antivenom. |
HostingRepository | PRIDE |
AnnounceDate | 2019-02-25 |
AnnouncementXML | Submission_2019-02-25_07:27:36.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Eduardo Kitano |
SpeciesList | scientific name: Naja annulifera; NCBI TaxID: 96794; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-31 02:17:16 | ID requested | |
⏵ 1 | 2019-02-25 07:27:38 | announced | |
Publication List
Silva-de-Fran, ç, a F, Villas-Boas IM, Serrano SMT, Cogliati B, Chudzinski SAA, Lopes PH, Kitano ES, Okamoto CK, Tambourgi DV, Naja annulifera Snake: New insights into the venom components and pathogenesis of envenomation. PLoS Negl Trop Dis, 13(1):e0007017(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Naja annulifera snake venom, toxins, inflammation, antivenom, LC-MS/MS, iBAQ |
Contact List
Solange M. T. Serrano |
contact affiliation | Special Laboratory of Applied Toxinology, Butantan Institute, São Paulo, Brazil |
contact email | solange.serrano@butantan.gov.br |
lab head | |
Eduardo Kitano |
contact affiliation | Butantan Institute |
contact email | eduardosh.kitano@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010962
- Label: PRIDE project
- Name: Naja annulifera Snake: New Insights into the Venom Components and Pathogenesis of Envenomation