PXD010961-2

PXD010961 is an original dataset announced via ProteomeXchange.

Title	“Overlapping pictures, proteomic study of the membrane components of signaling cascades in Botrytis cinerea controlled by phosphorylation”
Description	The phytopathogen Botrytis cinerea is a ubiquitous fungus with a high capacity to adapt its metabolism to different hosts and environmental conditions in order to deploy a variety of virulence and pathogenicity factors and develop a successful plant infection. Here we report the first comparative phosphomembranome study of B. cinerea, aimed to analyze the membrane phosphoproteins composition of the fungus and its changes under different phenotypical conditions induced by two different carbon sources as plant based elicitors: glucose and deproteinized tomato cell wall (TCW). Proteomics analysisof membrane phosphoproteins was carried out by mass spectrometry. A total of 1115 proteins were successfully identified. Further analyses showed significant differences in the phosphomembranome composition depending on the available carbon source: 64 proteins were exclusively identified or overexpressed when the fungus was cultured with glucose as a sole carbon source, and 243 proteins were exclusively identified or overexpressed with TCW. GO and KEGG enrichment and clustering interaction analysis revealed changes in the composition of phosphomembranome with increase of autophagy in glucose conditions and protein degradation process, unfolded protein response (UPR) and ribosome biogenesis in TCW conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:08:08.265.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francisco Fernández-Acero
SpeciesList	scientific name: Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea); NCBI TaxID: 332648;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-31 02:16:40	ID requested
1	2019-07-15 02:15:26	announced
⏵ 2	2024-10-22 04:08:11	announced	2024-10-22: Updated project metadata.

10.1038/s41598-019-46270-0;

Escobar-Ni, ñ, o A, Li, ñ, eiro E, Amil F, Carrasco R, Chiva C, Fuentes C, Blanco-Ulate B, Cantoral Fern, á, ndez JM, Sabid, ó E, Fern, á, ndez-Acero FJ, Proteomic study of the membrane components of signalling cascades of Botrytis cinerea controlled by phosphorylation. Sci Rep, 9(1):9860(2019) [pubmed]

curator keyword: Biological

submitter keyword: Botrytis cinerea, phosphomembranome, LC-MS/MS

Francisco Javier Fernández Acero
contact affiliation	Andalusian CenterforGrapeandGrapevineResearch,CeiA3,MarineandEnvironmentalSciencesFaculty,Laboratory of Microbiology,UniversityofCadiz (UCA),PuertoReal,11510Cádiz,Spain
contact email	franciscojavier.fernandez@uca.es
lab head
Francisco Fernández-Acero
contact affiliation	University of Cadiz
contact email	franciscojavier.fernandez@uca.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD010961

PRIDE project URI

• PRIDE
  1. PXD010961
     1. Label: PRIDE project
     2. Name: “Overlapping pictures, proteomic study of the membrane components of signaling cascades in Botrytis cinerea controlled by phosphorylation”