PXD010944-1

PXD010944 is an original dataset announced via ProteomeXchange.

Title	REX1 is the critical target of RNF12 in imprinted X chromosome inactivation in mice.
Description	In mice, imprinted X-chromosome inactivation (iXCI) of the paternal X in the pre-implantation embryo and extra-embryonic tissues is followed by X-reactivation in the epiblast precursors of the inner cell mass (ICM) of the blastocyst, to facilitate initiation of random XCI (rXCI) in all embryonic tissues. RNF12 is an E3 ubiquitin-ligase that plays a key role in XCI. RNF12 targets pluripotency protein REX1 for degradation to initiate rXCI in embryonic stem cells (ESCs) and loss of the maternal copy of Rnf12 leads to embryonic lethality due to iXCI failure. Here, we show that loss of Rex1 rescues the rXCI phenotype observed in Rnf12-/- ESCs, and that REX1 is the prime target of RNF12 in ESCs. Genetic ablation of Rex1 in Rnf12-/- mice rescues the Rnf12-/- iXCI phenotype, and results in viable and fertile Rnf12-/-:Rex1-/- female mice displaying normal iXCI and rXCI. Our results show that REX1 is the critical target of RNF12 in XCI.
HostingRepository	PRIDE
AnnounceDate	2018-11-15
AnnouncementXML	Submission_2018-11-15_14:09:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeroen Demmers
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-30 05:47:29	ID requested
⏵ 1	2018-11-15 14:09:25	announced
2	2018-12-04 03:59:43	announced	Updated project metadata.
3	2024-10-22 04:08:00	announced	2024-10-22: Updated project metadata.

Gontan C, Mira-Bontenbal H, Magaraki A, Dupont C, Barakat TS, Rentmeester E, Demmers J, Gribnau J, REX1 is the critical target of RNF12 in imprinted X chromosome inactivation in mice. Nat Commun, 9(1):4752(2018) [pubmed]

submitter keyword: REX1, RNF12, Mouse, SILAC, XCI, proteomics

Jeroen Demmers
contact affiliation	Proteomics Center, Erasmus MC, Rotterdam, the Netherlands
contact email	j.demmers@erasmusmc.nl
lab head
Jeroen Demmers
contact affiliation	Proteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands
contact email	j.demmers@erasmusmc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010944
     1. Label: PRIDE project
     2. Name: REX1 is the critical target of RNF12 in imprinted X chromosome inactivation in mice.