PXD010943-2

PXD010943 is an original dataset announced via ProteomeXchange.

Title	SWATH-MS Analysis of Gfi1-mutant bone marrow neutrophils
Description	This study investigates the proteomic alterations in bone marrow neutrophils isolated from 5-8 week old Gfi1+/-, Gfi1K403R/-, Gfi1R412X/-, and Gfi1R412X/R412X mice using the SWATH-MS technique. This dataset consists of 26 raw MS files, comprising 13 DDA (for spectral library generation)and 13 DIA (for SWATH) runs on a TripleTOF 5600 plus (SCIEX). Our findings revealed significant changes in the expression of neutrophil granule proteins and NADPH-oxidase complex components in Gfi1-mutant neutrophils.
HostingRepository	PRIDE
AnnounceDate	2020-04-07
AnnouncementXML	Submission_2020-06-05_04:05:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	H. Leighton Grimes
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-30 05:45:42	ID requested
1	2020-04-06 22:20:51	announced
⏵ 2	2020-06-05 04:05:45	announced	2020-06-05: Updated publication reference for PubMed record(s): 32494068.

Muench DE, Olsson A, Ferchen K, Pham G, Serafin RA, Chutipongtanate S, Dwivedi P, Song B, Hay S, Chetal K, Trump-Durbin LR, Mookerjee-Basu J, Zhang K, Yu JC, Lutzko C, Myers KC, Nazor KL, Greis KD, Kappes DJ, Way SS, Salomonis N, Grimes HL, Mouse models of neutropenia reveal progenitor-stage-specific defects. Nature, 582(7810):109-114(2020) [pubmed]

curator keyword: Technical, Biological

submitter keyword: Neutrophil, SCN, Gfi1, SWATH

Kenneth D. Greis
contact affiliation	UC Proteomics Laboratory, Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, Ohio, Unite States
contact email	greiskd@ucmail.uc.edu
lab head
H. Leighton Grimes
contact affiliation	Cincinnati Children's Hospital Medical Center
contact email	lee.grimes@cchmc.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD010943

PRIDE project URI

• PRIDE
  1. PXD010943
     1. Label: PRIDE project
     2. Name: SWATH-MS Analysis of Gfi1-mutant bone marrow neutrophils