PXD010938 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative proteomics using iodoTMT to identify changes in protein expression and cysteine oxidation in Leishmania mexicana |
Description | Two cell-lines of Leishmania mexicana were investigated using a TMT-based approach; wild-type and an attenuated H-line. Half of each sample was fully reduced and all cysteine residues were labelled with the cysteine-reactive iodoTMT reagent, while the other half was reacted with N-ethylmaleimide to block all free cysteines before the remaining reversibly modified cysteines were reduced and labelled with iodoTMT. This allowed direct comparisons to be made between the level of modified cysteines and the total cysteine count. |
HostingRepository | PRIDE |
AnnounceDate | 2018-11-23 |
AnnouncementXML | Submission_2018-11-23_08:49:20.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Aruna Prakash |
SpeciesList | scientific name: Leishmania mexicana MHOM/GT/2001/U1103; NCBI TaxID: 929439; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-30 01:22:38 | ID requested | |
⏵ 1 | 2018-11-23 08:49:20 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Comparative proteomics, Leishmania mexicana, TMT, cysteine oxidation |
Contact List
Richard Burchmore |
contact affiliation | Head of Proteomics at Glasgow Polyomics, College of Medical Veterinary and Life Sciences, University of Glasgow, UK |
contact email | richard.burchmore@glasgow.ac.uk |
lab head | |
Aruna Prakash |
contact affiliation | University of Glasgow |
contact email | a.s.prakash@dunelm.org.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010938
- Label: PRIDE project
- Name: Comparative proteomics using iodoTMT to identify changes in protein expression and cysteine oxidation in Leishmania mexicana