PXD010900 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | To invesigate the role of autophagy in Arabidopsis root cellular protein turnover and proteostasis |
Description | Protein turnover analysis using partial 15N labelling and protein abundance analysis using 15N spike-in were coupled to investiage the role of autophagy in protein degradation and proteostasis. Our findings show that dysfuncional autophagy due to loss-of-function autophagy components ATG5 and 11 lead to accumulation of protein targets with/without starvation stress. Further protein turnover analysis uncover a subgroup of proteins with retarded degradation. New selective autophagy protein targets involving glycolytic proteins were uncovered by this strategy. Furthermore, we found plasma membrane and cytoskeleton proteins show a general reduction, supporting the cross-interaction between autopahgy and vesicle trafficking pathway. Metabolomics changes in autophagy mutants due to a changed proteostasis was further investigated. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:38:36.554.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lei Li |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | 6520A Quadrupole Time-of-Flight LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-28 05:59:47 | ID requested | |
⏵ 1 | 2024-10-08 11:38:37 | announced | |
Publication List
10.1093/plcell/koac185; |
Li L, Lee CP, Ding X, Qin Y, Wijerathna-Yapa A, Broda M, Otegui MS, Millar AH, Defects in autophagy lead to selective in vivo changes in turnover of cytosolic and organelle proteins in Arabidopsis. Plant Cell, 34(10):3936-3960(2022) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Arabidopsis, 15N labelling, protein turnover, autophagy |
Contact List
Harvey Millar |
contact affiliation | ARC Centre of Excellence in Plant Energy Biology, M316, 35 Stirling Hwy, 6009, WA, Australia Faculty of Science, The University of Western Australia |
contact email | harvey.millar@uwa.edu.au |
lab head | |
Lei Li |
contact affiliation | College of Life Sciences, Nankai University, Tianjin 300071 |
contact email | lei.li@nankai.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010900
- Label: PRIDE project
- Name: To invesigate the role of autophagy in Arabidopsis root cellular protein turnover and proteostasis