PXD010856-1

PXD010856 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	mPOP SCoPE-MS Master Mix 20180824
Description	A major limitation to applying quantitative LC-MS/MS proteomics to small samples, such as single cells, are the losses incured during sample cleanup. To relieve this limitation, we developed a Minimal ProteOmic sample Preparation (mPOP) method for culture-grown mammalian cells. mPOP obviates cleanup and thus eliminates cleanup-related losses while expediting sample preparation and simplifying its automation. Bulk SILAC samples processed by mPOP or by conventional urea-based methods indicated that mPOP results in complete cell lysis and accurate relative quantification. We integrated mPOP lysis with the Single Cell ProtEomics by Mass Spectrometry (SCoPE-MS) sample preparation, and benchmarked the quantification of such samples on a Q-exactive instrument. The results demonstrate low noise and high technical reproducibility. Then, we FACS sorted single U-937, HEK-293, and mouse embryonic stem cells into 96-well plates and analyzed them by automated mPOP and SCoPE-MS. The quantified proteins enabled separating the single cells by cell-type and cell-division-cycle phase.
HostingRepository	MassIVE
AnnounceDate	2018-08-23
AnnouncementXML	Submission_2018-08-23_15:13:59.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Harrison Specht
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-23 14:57:43	ID requested
⏵ 1	2018-08-23 15:14:00	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: single cell, single-cell, SCoPE-MS, mPOP, low input, automated, lysis

submitter keyword: single cell, single-cell, SCoPE-MS, mPOP, low input, automated, lysis

Contact List

Nikolai Slavov
contact affiliation	Northeastern University
contact email	nslavov@alum.mit.edu
lab head
Harrison Specht
contact affiliation	Northeastern University
contact email	specht.h@husky.neu.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082841