PXD010846 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A dynamic view of the proteomic landscape during differentiation of ReNcell VM cells, an immortalized human neural progenitor line |
Description | The immortalized human ReNcell VM cell line represents a reproducible and easy-to-propagate cell culture system for studying the differentiation of neural progenitors. To better characterize the starting line and its differentiation, we assessed protein and phospho-protein levels over a two-week period during which ReNcell progenitors differentiated into neurons, astrocytes, and oligodendrocytes. Five of the datasets measured protein levels or states of phosphorylation based on tandem-mass-tag (TMT) mass spectrometry. Proteomic analysis revealed reproducible changes in pathways responsible for cytoskeletal rearrangement, cell phase transitions, neuronal migration, glial differentiation, neurotrophic signalling and extracellular matrix regulation. Proteomic data revealed accelerated differentiation in cells treated with the poly-selective CDK and GSK3 inhibitor kenpaullone or the HMG-CoA reductase inhibitor mevastatin, both of which have previously been reported to promote neural differentiation. These data provide in-depth information on the ReNcell progenitor state and on neural differentiation in the presence and absence of drugs, setting the stage for functional studies. |
HostingRepository | PRIDE |
AnnounceDate | 2019-01-07 |
AnnouncementXML | Submission_2019-01-07_07:55:58.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Matthew Berberich |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-23 06:51:53 | ID requested | |
⏵ 1 | 2019-01-07 07:55:58 | announced | |
2 | 2019-02-24 23:58:09 | announced | Updated publication reference for PubMed record(s): 30778261. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Neuron, Differentiation, LC-MS/MS, TMT, Phosphoproteomics, Orbitrap Fusion Lumos |
Contact List
Peter Sorger |
contact affiliation | Professor of Systems Pharmacology Head of Harvard Program in Therapeutic Science Harvard Medical School |
contact email | peter_sorger@hms.harvard.edu |
lab head | |
Matthew Berberich |
contact affiliation | Harvard Program In Therapeutic Science/Harvard Medical School |
contact email | matthew_berberich@hms.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010846
- Label: PRIDE project
- Name: A dynamic view of the proteomic landscape during differentiation of ReNcell VM cells, an immortalized human neural progenitor line