PXD010825-2

PXD010825 is an original dataset announced via ProteomeXchange.

Title	A photo-cleavable surfactant for top-down proteomics
Description	We report the discovery of a photo-cleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo) that can be rapidly degraded upon UV irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to SDS and is mass spectrometry (MS)-compatible. Azo-aided top-down proteomics allowed the detection of 100-fold more unique proteoforms and enabled the solubilization of membrane proteins for comprehensive characterization of post-translational modifications. Azo is simple to synthesize in large quantity for general use as an SDS replacement.
HostingRepository	PRIDE
AnnounceDate	2019-03-29
AnnouncementXML	Submission_2019-04-18_03:36:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	kyle brown
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	palmitoylated residue; phosphorylated residue; monohydroxylated residue; acetylated residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-20 02:51:27	ID requested
1	2019-03-29 12:24:16	announced
⏵ 2	2019-04-18 03:36:29	announced	Updated publication reference for PubMed record(s): 30988469.

Brown KA, Chen B, Guardado-Alvarez TM, Lin Z, Hwang L, Ayaz-Guner S, Jin S, Ge Y, A photocleavable surfactant for top-down proteomics. Nat Methods, 16(5):417-420(2019) [pubmed]

curator keyword: Biological

submitter keyword: LC-MSMS

Ying Ge
contact affiliation	University of Wisconsin-Madison
contact email	ying.ge@wisc.edu
lab head
kyle brown
contact affiliation	uw madison
contact email	kbrown33@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD010825

PRIDE project URI

• PRIDE
  1. PXD010825
     1. Label: PRIDE project
     2. Name: A photo-cleavable surfactant for top-down proteomics