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PXD010776-2

PXD010776 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass spectrometry evaluation of a neuroblastoma SH-SY5Y cell culture protocol
DescriptionCell line-based proteomics studies are susceptible to intrinsic biological variation that contributes to increasing false positive claims; most of the methods that follow these changes offer a limited understanding of the biological system. We applied a quantitative proteomic strategy (iTRAQ) to detect intrinsic protein variation across SH-SY5Y cell culture replicates. More than 95% of the quantified proteins presented a coefficient of variation (CV) <20% between biological replicates and the variable proteins, which included cytoskeleton, cytoplasmic and housekeeping proteins, are widely reported in proteomic studies. We recommend this approach as an additional quality control before starting any proteomic experiment.
HostingRepositoryPRIDE
AnnounceDate2018-08-17
AnnouncementXMLSubmission_2018-08-28_08:32:32.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJIMMY RODRIGUEZ
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiTRAQ4plex-116 reporter+balance reagent acylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-08-15 02:37:48ID requested
12018-08-17 04:23:06announced
22018-08-28 08:32:34announcedUpdated publication reference for PubMed record(s): 30145218.
32018-12-04 04:07:17announcedUpdated project metadata.
42024-10-22 04:48:18announced2024-10-22: Updated project metadata.
Publication List
Murillo JR, Pla I, Goto-Silva L, Nogueira FCS, Domont GB, Perez-Riverol Y, S, รก, nchez A, Junqueira M, Mass spectrometry evaluation of a neuroblastoma SH-SY5Y cell culture protocol. Anal Biochem, 559():51-54(2018) [pubmed]
Keyword List
submitter keyword: Cell culture, iTRAQ, quantitative proteomics, biological variation
Contact List
Magno Junqueira
contact affiliationProteomics Unit, Chemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro - RJ, Brazil
contact emailmagnojunqueira@iq.ufrj.br
lab head
JIMMY RODRIGUEZ
contact affiliationCenter of Excellence in Biological and Medical Mass Spectrometry, Biomedical Center D13, Lund University, 221 84 Lund, Sweden.
contact emailesneider007@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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