PXD010769 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | identification of the endogenous host targets of the Salmonella effectors SSeK1 and 3 |
Description | Strains of Salmonella utilise two distinct type three secretion systems to deliver effector proteins directly into host cells. The Salmonella effectors SseK1 and SseK3 are arginine glycosyltransferases that modify mammalian death domain containing proteins with N-acetyl glucosamine (GlcNAc) when overexpressed ectopically or as recombinant protein fusions. Here, we combined Arg-GlcNAc glycopeptide immunoprecipitation and mass spectrometry to identify host proteins GlcNAcylated by endogenous levels of SseK1 and SseK3 during Salmonella infection. We observed that SseK1 modified the mammalian signaling protein TRADD, but not FADD as previously reported. Overexpression of SseK1 greatly broadened substrate specificity, while ectopic co-expression of SseK1 and TRADD increased the range of modified arginine residues within the death domain of TRADD. In contrast, endogenous levels of SseK3 resulted in modification of the death domains of receptors of the mammalian TNF superfamily, TNFR1 and TRAILR, at residues Arg376 and Arg293 respectively. Structural studies on SseK3 showed that the enzyme displays a classic GT-A glycosyltransferase fold and binds UDP-GlcNAc in a narrow and deep cleft with the GlcNAc facing the surface. Together our data suggests that Salmonellae carrying sseK1 and sseK3 employ the glycosyltransferase effectors to antagonise different components of death receptor signaling. |
HostingRepository | PRIDE |
AnnounceDate | 2019-03-27 |
AnnouncementXML | Submission_2019-03-27_03:25:41.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nichollas Scott |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344; NCBI TaxID: 216597; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | complex glycosylation |
Instrument | Orbitrap Fusion Lumos; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-14 03:20:18 | ID requested | |
⏵ 1 | 2019-03-27 03:25:43 | announced | |
Publication List
Newson JPM, Scott NE, Yeuk Wah Chung I, Wong Fok Lung T, Giogha C, Gan J, Wang N, Strugnell RA, Brown NF, Cygler M, Pearson JS, Hartland EL, Effectors SseK1 and SseK3 Target Death Domain Proteins in the TNF and TRAIL Signaling Pathways. Mol Cell Proteomics, 18(6):1138-1156(2019) [pubmed] |
Keyword List
ProteomeXchange project tag: Glycoproteomics (B/D-HPP) |
curator keyword: Biological |
submitter keyword: Salmonella, glycosylation, infection, proteomics, glycopeptide enrichment |
Contact List
Elizabeth L Hartland |
contact affiliation | Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, Victoria, Australia |
contact email | elizabeth.hartland@hudson.org.au |
lab head | |
Nichollas Scott |
contact affiliation | University of Melbourne |
contact email | nichollas.scott@unimelb.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010769
- Label: PRIDE project
- Name: identification of the endogenous host targets of the Salmonella effectors SSeK1 and 3