PXD010747-1

PXD010747 is an original dataset announced via ProteomeXchange.

Title	SWATH proteomics reveals that reductants selectively disrupt collagen homeostasis in human dermal fibroblasts and modify growth factor-independent signalling through the MAPK/Akt pathway
Description	We have used an unbiased, label-free SWATH proteomic approach to quantitate the response of skin fibroblast cells to the reductant DTT in the presence or absence of the growth factor PDGF. Of the 4487 proteins that were identified, only 47 proteins showed a statistically significant change of 2-fold or more with reducing stress. Our data demonstrates that reducing stress results in the loss of a small subset of reductant-sensitive proteins, including the fibrillar collagens (COL1A1/2 and COL3A1) and the myopathy-associated bundling collagen COL6A1/2/3.
HostingRepository	PRIDE
AnnounceDate	2019-03-25
AnnouncementXML	Submission_2019-03-25_04:52:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adrian Brown
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-10 02:28:08	ID requested
⏵ 1	2019-03-25 04:52:31	announced

Carne NA, Bell S, Brown AP, M, ä, ä, tt, ä A, Flagler MJ, Benham AM, Reductive Stress Selectively Disrupts Collagen Homeostasis and Modifies Growth Factor-independent Signaling Through the MAPK/Akt Pathway in Human Dermal Fibroblasts. Mol Cell Proteomics, 18(6):1123-1137(2019) [pubmed]

curator keyword: Biological

submitter keyword: SWATH, redox signalling, skin fibroblasts, collagen, PDGF, MAPK

Adrian Paul Brown
contact affiliation	Department of Biosciences, Durham University, UK
contact email	a.p.brown@durham.ac.uk
lab head
Adrian Brown
contact affiliation	Durham University
contact email	a.p.brown@durham.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010747
     1. Label: PRIDE project
     2. Name: SWATH proteomics reveals that reductants selectively disrupt collagen homeostasis in human dermal fibroblasts and modify growth factor-independent signalling through the MAPK/Akt pathway