PXD010741-1

PXD010741 is an original dataset announced via ProteomeXchange.

Title	MHC I-restricted immunopeptidomics analysis of human monocyte-derived macrophages exposed to biological nanoparticles bearing influenza hemagglutinin
Description	In this study, primary human monocyte-derived macrophages were exposed to plant-derived virus-like particles (VLPs) bearing influenza A hemagglutinin (HA) or soluble influenza HA, as control. Immunopurified MHC class I-associated peptides were analysed by nano-flow high pressure liquid chromatography coupled to high-resolution dopant-assisted electrospray ionisation mass spectrometry. A total of 109 host-derived MHC I peptides were identified in the VLP-treated samples, two of which were also detected in controls. The peptides unique to VLP treatment were, on average, ~13 amino acid residues long, more basic and hydrophilic, and were mainly processed via proteolysis by matrix metalloproteinases and cathepsins. The proteins associated with these peptides were primarily involved in cellular, metabolic and regulatory processes and activated several pathways including inflammation stimulation and attenuation, response to stimuli, innate and adaptive immunity, clathrin-mediated endocytosis, protein synthesis and endo-lysosomal degradation. This study is the first report to describe the response of a primary human antigen-presenting cell to nanoparticulate vs. soluble antigen exposure from an immunopeptidomics point of view.
HostingRepository	PRIDE
AnnounceDate	2022-02-24
AnnouncementXML	Submission_2022-02-24_11:57:09.419.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Momar Ndao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-10 02:14:23	ID requested
⏵ 1	2022-02-24 11:57:09	announced
2	2023-11-14 08:49:38	announced	2023-11-14: Updated project metadata.

10.1038/S41541-019-0111-Y;

curator keyword: Biological, Biomedical

submitter keyword: Class I major histocompatibility complex (MHC), human antigenic peptides, monocyte-derived macrophage (MDM), proteomics, immunopeptidomics, high-resolution mass spectrometry, dopant-assisted electrospray ionisation (DA-ESI), virus-like particle (VLP)

Momar Ndao
contact affiliation	Infectious Diseases and Immunity in Global Health Program, Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada
contact email	momar.ndao@mcgill.ca
lab head
Momar Ndao
contact affiliation	McGill University
contact email	momar.ndao@mcgill.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD010741

PRIDE project URI

• PRIDE
  1. PXD010741
     1. Label: PRIDE project
     2. Name: MHC I-restricted immunopeptidomics analysis of human monocyte-derived macrophages exposed to biological nanoparticles bearing influenza hemagglutinin