PXD010723-1

PXD010723 is an original dataset announced via ProteomeXchange.

Title	Data in support of Proteomic analysis of synovial fluid from rheumatic arthritis and spondyloarthritis patients
Description	Objective. To analyse the pathogenesis of the diseases rheumatoid arthritis (RA) and spondyloarthritis (SpA) we investigated the protein composition in synovial fluid from the patients. Methods. Fifty-six synovial fluid (SF) were analysed with non-gel based proteomics from patients with RA (32) and SpA (24). Rheumatoid factor was measured in plasma, and cell-free DNA was measured in SF. Results. Two hundred proteins were quantified in the samples. The inflammatory proteins were more abundant in the RA group, specially proteins from neutrophil granulocytes. Cell-free DNA (cfDNA) in the SF was statistically associated with proteins that are known to form neutrophil extracellular traps (NETs). Plasma C-reactive protein (CRP), was correlated to other acute phase proteins in the SF. Minimal correlation was seen between acute phase proteins and proteins in NETs. Conclusions. The results show that in the synovial cavity in vivo neutrophils form NETs. This result sustains that neutrophils from RA patients are activated and are likely to undergo NETosis resulting in SF cfDNA specific of arthritis pathology with NETosis.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_06:53:46.039.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Allan Stensballe
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-08 05:39:19	ID requested
⏵ 1	2022-02-15 06:53:46	announced
2	2024-10-22 05:31:18	announced	2024-10-22: Updated project metadata.

Birkelund S, Bennike TB, Kastaniegaard K, Lausen M, Poulsen TBG, Kragstrup TW, Deleuran BW, Christiansen G, Stensballe A, Proteomic analysis of synovial fluid from rheumatic arthritis and spondyloarthritis patients. Clin Proteomics, 17():29(2020) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Proteomics, Synovial Fluid, Rheumatoid Arthritis, Ankylosing Spondylitis

Allan Stensballe
contact affiliation	laboratory for Medical Massspectrometry, Aalborg University, Denmark
contact email	as@hst.aau.dk
lab head
Allan Stensballe
contact affiliation	Aalborg University
contact email	as@hst.aau.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010723
     1. Label: PRIDE project
     2. Name: Data in support of Proteomic analysis of synovial fluid from rheumatic arthritis and spondyloarthritis patients