PXD010713-1

PXD010713 is an original dataset announced via ProteomeXchange.

Title	Bovine extracellular vesicle LC-MSMS C4PR_LIV
Description	The tick-borne protozoan parasites, Theileria annulata and T. parva are unique amongst intracellular eukaryotic pathogens because of their ability to induce a phenotype akin to transformation of the host cell. Infection with T. annulata, the causative agent of tropical theileriosis, is frequently fatal, via a cycle of neoplastic growth and tissue dissemination. Infected leukocytes become highly metastatic, forming foci of infected cells in multiple organs with subsequent destruction and disorganisation of the lymphoid system. Studies on extracellular vesicles (EV) have revealed that inter-cellular communication is critical for metastatic progression in cancer, and that the release of exosomes, a sub-set of EV, from tumour cells into the micro-environment induces pre-metastatic niche formation. Here we compared EV from a bovine lymphosarcoma cell line (BL20) and the same line infected in vitro with T. annulata (TBL20) by comparative mass spectrometry and miRNA profiling. Ingenuity Pathway Analysis revealed that many infection-associated proteins essential to migration and extracellular matrix digestion were up-regulated in EV from TBL20 cells compared with those from BL20 controls. Analysis of EV-associated miRNA revealed an altered repertoire of six host miRNA, each with a known role in tumour and/or infection biology. Focusing on bta-miR-181a and bta-miR-181b, we identified relevant mRNA targets and confirmed the interaction of bta-miR181a with intracellular adhesion molecule 1 (ICAM1). These data support a role for EV and their miRNA cargo in the pathobiology of Theileria infection.
HostingRepository	PRIDE
AnnounceDate	2018-11-04
AnnouncementXML	Submission_2018-11-04_15:54:18.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010713
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Deborah Simpson
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-07 06:32:40	ID requested
⏵ 1	2018-11-04 15:54:20	announced
2	2018-12-04 03:58:08	announced	Updated project metadata.

Gillan V, Simpson DM, Kinnaird J, Maitland K, Shiels B, Devaney E, Characterisation of infection associated microRNA and protein cargo in extracellular vesicles of Theileria annulata infected leukocytes. Cell Microbiol, 21(1):e12969(2019) [pubmed]

curator keyword: Biological

submitter keyword: Extracellular vesicles, Cow, Theileria annulata, LC-MSMS

Professor Eileen Devaney
contact affiliation	Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Glasgow G61 1QH
contact email	eileen.devaney@glasgow.ac.uk
lab head
Deborah Simpson
contact affiliation	University of Liverpool
contact email	dsimpson@liverpool.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010713

PRIDE project URI

• PRIDE
  1. PXD010713
     1. Label: PRIDE project
     2. Name: Bovine extracellular vesicle LC-MSMS C4PR_LIV