PXD010697-1

PXD010697 is an original dataset announced via ProteomeXchange.

Title	Sleep-wake cycles drive daily dynamics of synaptic phosphorylation
Description	The circadian clock drives daily changes of physiology, including sleep-wake cycles, by regulating transcription, protein abundance and function. Circadian phosphorylation controls cellular processes in peripheral organs, but little is known about its role in brain function and synaptic activity. We applied advanced quantitative phosphoproteomics to mouse forebrain synaptoneurosomes isolated across 24h, accurately quantifying almost 8,000 phosphopeptides. Remarkably, half of the synaptic phosphoproteins, including numerous kinases, had large-amplitude rhythms peaking at rest-activity and activity-rest transitions. Bioinformatic analyses revealed global temporal control of synaptic function via phosphorylation, including synaptic transmission, cytoskeleton reorganization and excitatory/inhibitory balance. Remarkably, sleep deprivation abolished 98% of all phosphorylation cycles in synaptoneurosomes, indicating that sleep-wake cycles rather than circadian signals are main drivers of synaptic phosphorylation, responding to both sleep and wake pressures.
HostingRepository	PRIDE
AnnounceDate	2019-10-14
AnnouncementXML	Submission_2019-10-14_01:44:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-06 06:15:40	ID requested
⏵ 1	2019-10-14 01:45:00	announced
2	2019-10-16 00:08:51	announced	2019-10-16: Updated publication reference for PubMed record(s): 31601740, 31601739.

Noya SB, Colameo D, Br, ü, ning F, Spinnler A, Mircsof D, Opitz L, Mann M, Tyagarajan SK, Robles MS, Brown SA, The forebrain synaptic transcriptome is organized by clocks but its proteome is driven by sleep. Science, 366(6462):(2019) [pubmed]

curator keyword: Biological

submitter keyword: mass spectrometry, phosphoproteomics, phosphorylation, synaptoneurosome, circadian, sleep, EasyPhos

Maria S Robles
contact affiliation	Institute of Medical Psychology, LMU Munich, Germany
contact email	charo.robles@med.uni-muenchen.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD010697

PRIDE project URI

• PRIDE
  1. PXD010697
     1. Label: PRIDE project
     2. Name: Sleep-wake cycles drive daily dynamics of synaptic phosphorylation