PXD010670-1

PXD010670 is an original dataset announced via ProteomeXchange.

Title	A non-canonical BRD9-containing BAF chromatin remodeling complex regulates naïve pluripotency in mouse embryonic stem cells
Description	The mammalian BRG1-associated factors (BAF) complex is a multi-subunit chromatin remodeling complex that is an important component of the embryonic stem cell (ESC) transcriptional regulatory network. However, the role of individual subunits in BAF complex targeting and function needs to be elucidated. Here, we find that the Bromodomain containing protein 9 (BRD9) defines a smaller, non-canonical BAF complex in mouse ESCs that is distinct from the canonical embryonic stem cell BAF (esBAF) and the polybromo-associated BAF (PBAF) complexes. This BRD9-containing BAF complex, or BBAF complex, uniquely incorporates the BRD4-interacting chromatin remodeling associated protein-like (BICRAL) or its paralog BICRA and lacks several esBAF subunits including BAF47, ARID1A and BAF57. We demonstrate that BBAF and esBAF complexes are targeted to different features of the genome and are co-bound with different sets of pluripotency transcription factors. Specifically, BBAF complexes co-localize with key regulators of naïve pluripotency, KLF4 and Sp5, on the genome. Consistent with this, we provide evidence that BBAF’s specific function is to regulate the transcription of genes involved in the maintenance of naïve pluripotency, including Nanog and Prdm14. Additionally, we show that BRD9 is displaced from chromatin by the selective BRD9 bromodomain inhibitor, I-BRD9, and that this leads to changes in target gene expression. Together, our results provide evidence for the identification of a new BAF complex, and demonstrate functionally specific roles for BAF complex assemblies in maintaining the transcriptional network of pluripotency.
HostingRepository	PRIDE
AnnounceDate	2018-10-18
AnnouncementXML	Submission_2018-10-18_00:31:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Moresco
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-03 02:02:43	ID requested
⏵ 1	2018-10-18 00:31:31	announced
2	2019-02-07 03:51:39	announced	Updated publication reference for PubMed record(s): 30510198.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: BAF, Chromatin remodeling

Diana C Hargreaves
contact affiliation	Molecular and Cellular Biology Laboratory, Salk Institute for Biological Studies, La Jolla California 92037 USA
contact email	dhargreaves@salk.edu
lab head
James Moresco
contact affiliation	The Salk Institute for Biological Studies
contact email	jmoresco@salk.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010670
     1. Label: PRIDE project
     2. Name: A non-canonical BRD9-containing BAF chromatin remodeling complex regulates naïve pluripotency in mouse embryonic stem cells