PXD010663-1

PXD010663 is an original dataset announced via ProteomeXchange.

Title	Bacteria Associated with Russian Wheat Aphid (Diuraphis noxia) Enhance Aphid Virulence to Wheat
Description	Phenotypic responses to biotic stresses are often studied as the interactions between two species; however, in the phytobiome, these responses frequently result from complex interactions involving several organisms. Here, we show that variation in chlorosis caused by Russian wheat aphid (Diuraphis noxia) feeding is determined, in part, by aphid-associated bacteria. Proteomic analysis of fluids injected into a sterile medium by the aphid during feeding indicate that 99% of the proteins are of bacterial origin. Of these, the greatest proportion are produced by bacteria in the order Enterobacteriales. Bacteria from five genera in four families that have the capacity to produce these proteins were isolated directly from aphids as well as from wheat leaves only after D. noxia feeding. By themselves or in combination, these bacteria were not virulent to wheat, even at high inoculum levels. Metagenomic analysis showed that the same five D. noxia-associated genera dominated the non-Buchnera component of the aphid microbiome, and that representation of these genera was reduced in aphids from colonies established after isolation of newborn nymphs from their mothers prior to feeding (‘isolated’ aphids). Isolation or treatment with antibiotics reduced bacterial numbers, and these aphids caused less feeding damage on wheat than non-isolated or non-antibiotic treated aphids. Our data show that bacterial proteins are a significant component of Russian wheat aphid saliva, that the bacteria producing these proteins are associated with aphids and plants fed upon by aphids, and that these aphid-associated bacteria facilitate aphid virulence to wheat.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:16:46.030.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010663
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kevin Howe
SpeciesList	scientific name: Diuraphis noxia; NCBI TaxID: 143948;
ModificationList	Oxidation
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-02 02:39:57	ID requested
⏵ 1	2024-10-07 10:16:46	announced

10.6019/PXD010663;

curator keyword: Metaproteomics, Biological

submitter keyword: Aphid saliva | Proteome | Enterobacteriaceae | Multitrophic interactions | Microbiome | Phytobiome

Michelle Heck
contact affiliation	USDA-ARS, Cornell University, Boyce Thompson Institute
contact email	mlc68@cornell.edu
lab head
Kevin Howe
contact affiliation	USDA-ARS
contact email	kjh46@cornell.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010663
     1. Label: PRIDE project
     2. Name: Bacteria Associated with Russian Wheat Aphid (Diuraphis noxia) Enhance Aphid Virulence to Wheat