PXD010612-1

PXD010612 is an original dataset announced via ProteomeXchange.

Title	CIP2A negatively regulates Th17 differentiation
Description	Interleukin 17 (IL-17) producing T helper 17 (Th17) cells are critical drivers of pathogenesis in a variety of autoimmune and inflammatory diseases. Strategies to mitigate excessive Th17 response thus remain an attractive target for immunotherapies. Here we report that Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A) regulates IL-17 production by Th17 cells in human and mouse. Using CIP2A knock-out (KO) mice and siRNA-mediated CIP2A silencing in human primary CD4+ T cells, we demonstrated that CIP2A silencing results in a significant increase in IL-17 production. Interestingly, CIP2A deficient Th17 cells were characterized by increased strength and duration of STAT3 (Y705) phosphorylation. Genome-wide gene expression profile as well as the p-STAT3 (Y705) interactome of CIP2A deficient Th17 cells identified that CIP2A regulates the strength of the interaction between Acylglycerol kinase (AGK) and STAT3, and thereby, modulates STAT3 phosphorylation as well as expression of IL-17 in Th17 cells. Our results uncover the physiological function of CIP2A in Th17 cells and provides new opportunities for therapeutic intervention in Th17 cell mediated diseases.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_12:10:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Santosh Bhosale
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-30 02:29:19	ID requested
⏵ 1	2020-05-26 12:10:20	announced
2	2024-10-22 05:04:56	announced	2024-10-22: Updated project metadata.

Khan MM, Ullah U, Khan MH, Kong L, Moulder R, V, ä, likangas T, Bhosale SD, Komsi E, Rasool O, Chen Z, Elo LL, Westermarck J, Lahesmaa R, CIP2A Constrains Th17 Differentiation by Modulating STAT3 Signaling. iScience, 23(3):100947(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Th17 cell differentiation, STAT3 phosphorylation

Riitta Lahesmaa
contact affiliation	Academy Professor, Turku Centre for Biotechnology, University of Turku and Abo Akademi University, Turku, Finland
contact email	rilahes@utu.fi
lab head
Santosh Bhosale
contact affiliation	Turku Centre for Biotechnology, Turku, Finland
contact email	sadibh@utu.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD010612

PRIDE project URI

• PRIDE
  1. PXD010612
     1. Label: PRIDE project
     2. Name: CIP2A negatively regulates Th17 differentiation