PXD010582 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Maize AF F24 Shotgun Proteomics |
Description | Ustilago maydis is a biotrophic fungus causing corn smut disease in maize. To downregulate immune responses and promote host colonization, U. maydis secretes a set of effector proteins into the plant apoplast. An effector essential for U. maydis virulence is Pit2, an inhibitor of papain-like cysteine proteases (PLCPs). Pit2 virulence function relies on a 14 amino acids motif (PID14). While sequence of the Pit2 effector is highly diverse amongst related pathogen species, the PID14 motif is highly conserved. Interestingly, synthetic PID14 peptides act more efficiently as PLCP inhibitors than the full-length Pit2 effector. In line with this finding, mass spectrometry showed processing of Pit2 by maize PLCPs, which releases an inhibitory core motif of the PID14. Mutational analysis demonstrated that two residues of the released inhibitor peptide are essential for Pit2 function and, consequently, for U. maydis virulence. Based on these findings, we propose a model, in which the Pit2 effector functions as a decoy: Pit2 represents a favorable substrate for apoplastic PLCPs, which are central hubs of the maize immune system. Processing of Pit2 releases the inhibitor peptide, which in turn efficiently blocks PLCPs to modulate host immunity. |
HostingRepository | PRIDE |
AnnounceDate | 2019-02-25 |
AnnouncementXML | Submission_2019-04-12_01:02:48.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fatih Demir |
SpeciesList | scientific name: Zea mays (Maize); NCBI TaxID: 4577; |
ModificationList | acetylated residue |
Instrument | maXis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-07-27 00:23:04 | ID requested | |
1 | 2019-02-25 06:25:35 | announced | |
⏵ 2 | 2019-04-12 01:02:49 | announced | Updated publication reference for PubMed record(s): 30952847. |
Publication List
Misas Villamil JC, Mueller AN, Demir F, Meyer U, Ö, kmen B, Schulze H, ü, ynck J, Breuer M, Dauben H, Win J, Huesgen PF, Doehlemann G, A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif. Nat Commun, 10(1):1576(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Maize Proteomics F24 PLCPs Apoplastic Fluid |
Contact List
Pitter Florian Huesgen |
contact affiliation | Forschungszentrum Juelich ZEA-3 Analytics Wilhelm-Johnen-Str D-52425 Juelich Germany |
contact email | p.huesgen@fz-juelich.de |
lab head | |
Fatih Demir |
contact affiliation | Forschungszentrum Juelich ZEA-3 Analytics Wilhelm-Johnen-Str D-52425 Juelich |
contact email | f.demir@fz-juelich.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010582
- Label: PRIDE project
- Name: Maize AF F24 Shotgun Proteomics