PXD010580-1

PXD010580 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis thaliana Col-0 pgm1 spsa1 highlight and chilling temperatures proteins LC-MS/MS
Description	Abiotic stress exposure of plants induces metabolic reprogramming which is tightly regulated by signalling cascades connecting transcriptional with translational and metabolic regulation. Complexity of such interconnected metabolic networks impedes the functional understanding of molecular plant stress response compromising the design of breeding strategies and biotechnological processes. Thus, defining a molecular network to enable the prediction of a plant’s stress mode promises to promote the understanding of stress responsive biochemical regulation and its technological application. Arabidopsis wild type plants and two mutant lines with deficiency in sucrose or starch metabolism were grown under ambient and cold/high light stress conditions. Stress-induced dynamics of the primary metabolome and the proteome were quantified in a mass spectrometry-based high-throughput experiment. Wild type data were used to train a machine learning algorithm to classify mutant lines under control and stress conditions. Multivariate analysis and classification identified a module consisting of 23 proteins enabling the reliable prediction of coupled temperature and light stress conditions. 18 of these 23 proteins displayed putative protein-protein interactions connecting transcriptional regulation with regulation of primary and secondary metabolism under stress. The identified stress-responsive core module provides evidence for predictability of complex biochemical regulation during environmental fluctuation.
HostingRepository	PRIDE
AnnounceDate	2018-11-06
AnnouncementXML	Submission_2018-11-06_08:19:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lisa Fürtauer
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-26 09:10:31	ID requested
⏵ 1	2018-11-06 08:19:11	announced
2	2018-12-04 01:40:49	announced	Updated project metadata.
3	2024-10-22 04:36:30	announced	2024-10-22: Updated project metadata.

F, ü, rtauer L, Pschenitschnigg A, Scharkosi H, Weckwerth W, N, ä, gele T, Combined multivariate analysis and machine learning reveals a predictive module of metabolic stress response in Arabidopsis thaliana. Mol Omics, 14(6):437-449(2018) [pubmed]

submitter keyword: Arabidopsis thaliana, pgm1, spsa1, abiotic stress, combined stress,metabolic reprogramming

mass spectrometry

machine learning

Thomas Nägele
contact affiliation	Ludwig-Maximilians-Universität Munich / Germany Department Biology I Plant Evolutionary Cell Biology
contact email	thomas.naegele@lmu.de
lab head
Lisa Fürtauer
contact affiliation	LMU Munich
contact email	Lisa.Fuertauer@lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010580

PRIDE project URI

• PRIDE
  1. PXD010580
     1. Label: PRIDE project
     2. Name: Arabidopsis thaliana Col-0 pgm1 spsa1 highlight and chilling temperatures proteins LC-MS/MS