PXD010579 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Stable-protein Pair Analysis as A Novel Strategy to Identify Proteomic Signatures: Application to Seminal Plasma From Infertile Patients. |
Description | Our aim was to define seminal plasma proteome signatures of infertile patients categorized according to their seminal parameters using TMT-LC-MS/MS. To that extent, quantitative proteomic data was analyzed following two complementary strategies: (i) the conventional approach based on standard statistical analyses of relative protein quantification values; and (ii) a novel strategy focused on establishing stable-protein pairs. By conventional analyses, the abundance of some seminal plasma proteins was found to be positively correlated with sperm concentration. However, this correlation was not found for all the peptides within a specific protein, bringing to light the high heterogeneity existing in the seminal plasma proteome due to both the proteolytic fragments and/or the post-translational modifications. This issue was overcome by conducting the novel stable-protein pairs analysis proposed herein. A total of 182 correlations comprising 24 different proteins were identified in the normozoospermic-control population, while this proportion was drastically reduced in infertile patients with altered seminal parameters (18 in patients with reduced sperm motility, 0 in patients with low sperm concentration and 3 in patients with no sperm in the ejaculate). These results suggest the existence of multiple etiologies causing the same alteration in seminal parameters. Additionally, the repetition of the stable-protein pair analysis in the control group by adding the data from a single patient at a time enabled to identify alterations in the stable-protein pairs profile of individual patients with altered seminal parameters. These results suggest potential underlying pathogenic mechanisms in individual infertile patients, and might open up a window to its application in the personalized diagnostic of male infertility. |
HostingRepository | PRIDE |
AnnounceDate | 2019-02-25 |
AnnouncementXML | Submission_2019-02-24_23:57:01.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD010579 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Rafael Oliva |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex; Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-07-26 08:42:25 | ID requested | |
⏵ 1 | 2019-02-24 23:57:02 | announced | |
2 | 2019-03-21 04:52:58 | announced | Updated publication reference for PubMed record(s): 30518674. |
Publication List
Barrachina F, Jodar M, Delgado-Due, ñ, as D, Soler-Ventura A, Estanyol JM, Mallofr, é C, Ballesc, à JL, Oliva R, Stable-protein Pair Analysis as A Novel Strategy to Identify Proteomic Signatures: Application To Seminal Plasma From Infertile Patients. Mol Cell Proteomics, 18(Suppl 1):S77-S90(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Male infertility, seminal plasma, proteomics, TMT, stable-protein pairs |
Contact List
Rafael Oliva Virgili |
contact affiliation | Genetics Unit, Department of Biomedical Sciences, Faculty of Medicine, Casanova 143, 08036 Barcelona, Spain |
contact email | roliva@ub.edu |
lab head | |
Rafael Oliva |
contact affiliation | Director Molecular Biology of Reproduction and Development research Group, Genetics Unit, Faculty of Medicine, University of Barcelona, Institut d'Investigacions Biomèdiques August Pi iSunyer (IDIBAPS), and Hospital Clinic, Barcelona, Spain. |
contact email | roliva@ub.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010579
- Label: PRIDE project
- Name: Stable-protein Pair Analysis as A Novel Strategy to Identify Proteomic Signatures: Application to Seminal Plasma From Infertile Patients.