PXD010555 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Impact of oligoarginine peptides in ribosome composition and chromatin proteomes |
Description | Expanded intronic GGGGCC repeats in C9ORF72 produce several dipeptides, from which the two that contain arginine, (PR)n and (GR)n, accumulate at nucleoli and kill cells. While this toxicity plays an important role in ALS pathogenesis, its mechanism remains unknown. We here show that PR dipeptides bind avidly to DNA and RNA, so that any cellular reaction involving nucleic acids is impaired by the dipeptides. Consistently, PR-induced cell death can be rescued by addition of non-coding oligonucleotides. Interestingly, the effects of PR dipeptides are to a large extent mimicked by protamine, a sperm-specific Arg-rich protein, and the toxicity of either protamine or PR dipeptides is rescued by the anticoagulant heparin. We propose that the generalized coating of nucleic acids by Arg-rich peptides accounts for the toxicity of C9ORF72 mutations in ALS. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-12 |
AnnouncementXML | Submission_2021-04-12_08:12:39.015.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Eduardo Zarzuela |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Q Exactive; maXis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-07-25 02:53:12 | ID requested | |
⏵ 1 | 2021-04-12 08:12:39 | announced | |
2 | 2024-10-22 05:21:01 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: ALS, peptides, repeats, proteomics |
Contact List
Oscar Fernández Capetillo |
contact affiliation | Genomic Instability Group,Spanish National Cancer Research Center (CNIO) |
contact email | ofernandez@cnio.es |
lab head | |
Eduardo Zarzuela |
contact affiliation | Centro Nacional de Investigaciones Oncológicas |
contact email | eduzarfer@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD010555
- Label: PRIDE project
- Name: Impact of oligoarginine peptides in ribosome composition and chromatin proteomes