PXD010519-2

PXD010519 is an original dataset announced via ProteomeXchange.

Title	Plant-derived virus-like particle vaccines drive cross-presentation of influenza A hemagglutinin peptides by human monocyte-derived macrophages
Description	A growing body of evidence supports the importance of T cell responses to protect against severe influenza, promote viral clearance and ensure long-term immunity. Plant-derived virus-like particle (VLP) vaccines bearing influenza hemagglutinin (HA) have been shown to elicit strong humoral and CD4+ T cell responses in both pre-clinical and clinical studies. To better understand the immunogenicity of theses vaccines, we tracked the intracellular fate of a model HA (A/California/07/2009 H1N1) in human monocyte-derived macrophages (MDMs) following delivery either as VLPs (H1-VLP) or in soluble form. High-resolution tandem mass spectrometry identified 131 HA-derived peptides associated with MHC I in the H1-VLP-treated MDMs. Together with immunostaining and microscopy results, these data suggest that HA delivery to antigen-presenting cells on plant-derived VLPs facilitates antigen uptake, endosomal processing and cross-presentation. These observations may help explain the broad and cross-reactive immune responses generated by these vaccines.
HostingRepository	PRIDE
AnnounceDate	2019-05-28
AnnouncementXML	Submission_2019-05-29_04:06:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Momar Ndao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-20 06:24:08	ID requested
1	2019-05-28 06:39:39	announced
⏵ 2	2019-05-29 04:07:01	announced	Updated publication reference for PubMed record(s): 31123605.
3	2024-10-22 04:47:06	announced	2024-10-22: Updated project metadata.

Makarkov AI, Golizeh M, Ruiz-Lancheros E, Gopal AA, Costas-Cancelas IN, Chierzi S, Pillet S, Charland N, Landry N, Rouiller I, Wiseman PW, Ndao M, Ward BJ, Plant-derived virus-like particle vaccines drive cross-presentation of influenza A hemagglutinin peptides by human monocyte-derived macrophages. NPJ Vaccines, 4():17(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: influenza, plant-derived vaccine, virus-like particle, cross-presentation, cell-mediated immunity, human monocyte-derived macrophage, major histocompatibility complex class I, high-resolution mass spectrometry

Brian J. Ward
contact affiliation	Infectious Diseases and Immunity in Global Health Program, Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada
contact email	brian.ward@mcgill.ca
lab head
Momar Ndao
contact affiliation	McGill University
contact email	momar.ndao@mcgill.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD010519

PRIDE project URI

• PRIDE
  1. PXD010519
     1. Label: PRIDE project
     2. Name: Plant-derived virus-like particle vaccines drive cross-presentation of influenza A hemagglutinin peptides by human monocyte-derived macrophages