PXD010494-2

PXD010494 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS EThcD of MERS-CoV and SARS-CoV S N-linked glycopeptides from recombinant spike ectodomains and whole MERS virions
Description	Recent outbreaks of severe acute respiratory syndrome and Middle-East respiratory syndrome along with the threat of a future coronavirus pandemic underscore the importance of finding ways to neutralize these viruses. The trimeric spike transmembrane glycoprotein S mediates entry into host cells and is the major target of neutralizing antibodies. To understand the humoral immune response elicited upon natural infections with coronaviruses, we structurally characterized the SARS-CoV and MERS-CoV S glycoproteins in complex with neutralizing antibodies isolated from human survivors using cryo electron microscopy and characterized the site-specific N-linked glycan profile of the recombinant S proteins with LC-MS/MS using EThcD fragmentation. Although the two antibodies studied blocked attachment to the host cell receptor, only the anti-SARS-CoV S antibody triggered premature fusogenic conformational changes via receptor functional mimicry. These results provide a structural framework for understanding coronavirus neutralization by human antibodies and shed light on the coronavirus fusion activation pathway which appears to take place through a receptor-driven ratcheting mechanism.
HostingRepository	PRIDE
AnnounceDate	2019-05-01
AnnouncementXML	Submission_2019-05-02_07:07:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joost Snijder
SpeciesList	scientific name: Severe acute respiratory syndrome-related coronavirus; NCBI TaxID: 694009; scientific name: Middle East respiratory syndrome-related coronavirus; NCBI TaxID: 1335626;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-19 01:53:19	ID requested
1	2019-05-01 10:01:45	announced
⏵ 2	2019-05-02 07:08:30	announced	Updated publication reference for PubMed record(s): 30712865.
3	2024-10-22 04:51:46	announced	2024-10-22: Updated project metadata.

Walls AC, Xiong X, Park YJ, Tortorici MA, Snijder J, Quispe J, Cameroni E, Gopal R, Dai M, Lanzavecchia A, Zambon M, Rey FA, Corti D, Veesler D, Unexpected Receptor Functional Mimicry Elucidates Activation of Coronavirus Fusion. Cell, 176(5):1026-1039.e15(2019) [pubmed]

submitter keyword: MERS, SARS, N-linked glycosylation, EThcD

David Veesler
contact affiliation	Department of Biochemistry, University of Washington, Seattle WA USA
contact email	dveesler@uw.edu
lab head
Joost Snijder
contact affiliation	University of Washington
contact email	joost.snijder@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD010494

PRIDE project URI

• PRIDE
  1. PXD010494
     1. Label: PRIDE project
     2. Name: LC-MS/MS EThcD of MERS-CoV and SARS-CoV S N-linked glycopeptides from recombinant spike ectodomains and whole MERS virions