PXD010456 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Sialo-glycoproteome of Human lung cancer cell by LC-MSMS |
Description | Alterations in protein glycosylation, especially the terminal sialylation, are closely correlated with physiological and pathological regulation. Due to the low ionization efficiency of sialo-glycopeptides and frequently observed dissociation of sialic acid residues during mass spectrometry analysis, tools to enrich the sialo-glycopeptides are essential to enhance detection sensitivity and identification efficiency in sialo-glycoproteomics. In this study, we present the first application of zwitterionic hydrophilic interaction chromatography (ZIC-cHILIC) material in StageTip for simultaneous enrichment and fractionation of intact glycopeptides at proteome scale. With the demonstrated enrichment specificity and identification depth, the stepwise-ZIC-cHILIC can be an efficient enrichment method for the discovery of glycosylation sites and native glycotope for many sample types. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-25 |
AnnouncementXML | Submission_2022-02-24_17:37:51.034.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD010456 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Yi-Ju Chen |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Deamidated; Oxidation; monohydroxylated residue; deaminated residue; Carbamidomethyl; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-07-17 04:14:23 | ID requested | |
⏵ 1 | 2022-02-24 17:37:51 | announced | |
Publication List
Chen YJ, Yen TC, Lin YH, Chen YL, Khoo KH, Chen YJ, ZIC-cHILIC-Based StageTip for Simultaneous Glycopeptide Enrichment and Fractionation toward Large-Scale N-Sialoglycoproteomics. Anal Chem, 93(48):15931-15940(2021) [pubmed] |
Keyword List
submitter keyword: glycoproteome, sialo-glycoproteome, human lung cancer |
Contact List
Yu-Ju Chen |
contact affiliation | Institute of Chemistry, Academia Sinica |
contact email | yujuchen@gate.sinica.edu.tw |
lab head | |
Yi-Ju Chen |
contact affiliation | Academia Sinica |
contact email | tp7249@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010456
- Label: PRIDE project
- Name: Sialo-glycoproteome of Human lung cancer cell by LC-MSMS