PXD010404 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A combination of proteomic approaches identifies a panel of circulating extracellular vesicle proteins related to the risk of suffering cardiovascular disease in obese patients |
Description | Plasma-derived extracellular vesicles (EVs) have been extensively described as putative biomarkers in different diseases. More precisely, increased levels of EVs subpopulations are well-known to associate with obesity. The goal of this study was to identify EVs-derived biomarkers in plasma from obese patients in order to predict the development of pathological events associated with obesity. The study was performed combining two different proteomic approaches (2D-DIGE and label-free LC-MS/MS). Samples were obtained from 22 obese patients, with no associated comorbidities, and their lean-matched controls. EVs were isolated following a serial ultracentrifugation protocol. We detected 22 and 23 different regulated protein features from 2D-DIGE and label free LC-MS/MS respectively. Most of the differentially regulated proteins identified were involved in the coagulation and complement cascade. Interestingly, there was a clear up-regulation of complement C3, complement C4 and fibrinogen in obese patients following both approaches, which correlates with a pro-inflammatory and prothrombotic state of those individuals. On the other hand, we showed a down-regulation of adiponectin leading to an increased risk of suffering cardiovascular diseases. Our results suggest the relevance of considering plasma-derived-EVs proteins as a source of potential biomarkers for the development of atherothrombotic events in obesity. |
HostingRepository | PRIDE |
AnnounceDate | 2018-12-17 |
AnnouncementXML | Submission_2018-12-17_05:54:50.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | María Núñez |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-07-12 07:38:13 | ID requested | |
⏵ 1 | 2018-12-17 05:54:51 | announced | |
Publication List
Barrachina MN, Sueiro AM, Casas V, Izquierdo I, Hermida-Nogueira L, Guiti, á, n E, Casanueva FF, Abi, á, n J, Carrascal M, Pardo M, Garc, í, a Á, A Combination of Proteomic Approaches Identifies A Panel of Circulating Extracellular Vesicle Proteins Related to the Risk of Suffering Cardiovascular Disease in Obese Patients. Proteomics, 19(1-2):e1800248(2019) [pubmed] |
Keyword List
curator keyword: Technical, Biological |
submitter keyword: Extracellular vesicles, Obesity, Plasma, Biomarkers, 2D-DIGE, Label-Free Quantitation |
Contact List
Maria N. Barrachina |
contact affiliation | Platelet Proteomics Group. Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), Universidade Santiago de Compostela; Instituto de Investigación Sanitaria de Santiago (IDIS). |
contact email | maria.nunez@usc.es |
lab head | |
María Núñez |
contact affiliation | University of Santiago de Compostela |
contact email | maria.nunez@usc.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010404
- Label: PRIDE project
- Name: A combination of proteomic approaches identifies a panel of circulating extracellular vesicle proteins related to the risk of suffering cardiovascular disease in obese patients