PXD010396-1

PXD010396 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Ubi complex characterization .
Description	Ubiquinone (UQ) is a polyprenylated lipid that is conserved from bacteria to humans and is crucial to cellular respiration. How the cell orchestrates the efficient synthesis of UQ, which involves the modification of extremely hydrophobic substrates by multiple sequential enzymes, remains an unresolved issue. Here, we demonstrate that seven Ubi proteins form the Ubi complex, a stable metabolon that catalyzes the last six reactions of the UQ biosynthetic pathway in Escherichia coli. In addition to five Ubi enzymes, the 1 MDa Ubi complex contains the newly identified UbiJ and UbiK proteins that we show to be involved in the binding of UQ intermediates and the structuration of the multiprotein complex. We also develop an in vitro assay and show that UQ biosynthesis occurs in the cytoplasmic fraction of E. coli, from which we purify the Ubi complex. Collectively, our work documents a rare case of stable metabolon and revisits the current thinking that the hydrophobic intermediates of the UQ pathway are metabolized in association with the membrane.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:15:58.469.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010396
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-12 02:26:25	ID requested
⏵ 1	2024-10-07 10:15:59	announced

Publication List

10.6019/PXD010396;
10.1016/j.chembiol.2018.12.001;
Hajj Chehade M, Pelosi L, Fyfe CD, Loiseau L, Rascalou B, Brugi, è, re S, Kazemzadeh K, Vo CD, Ciccone L, Aussel L, Cout, é Y, Fontecave M, Barras F, Lombard M, Pierrel F, A Soluble Metabolon Synthesizes the Isoprenoid Lipid Ubiquinone. Cell Chem Biol, 26(4):482-492.e7(2019) [pubmed]

10.6019/PXD010396;

10.1016/j.chembiol.2018.12.001;

Hajj Chehade M, Pelosi L, Fyfe CD, Loiseau L, Rascalou B, Brugi, è, re S, Kazemzadeh K, Vo CD, Ciccone L, Aussel L, Cout, é Y, Fontecave M, Barras F, Lombard M, Pierrel F, A Soluble Metabolon Synthesizes the Isoprenoid Lipid Ubiquinone. Cell Chem Biol, 26(4):482-492.e7(2019) [pubmed]

Keyword List

curator keyword: Biological
submitter keyword: ubiquinone, multiprotein complex,E. coli

curator keyword: Biological

submitter keyword: ubiquinone, multiprotein complex,E. coli

Contact List

Virginie Brun
contact affiliation	EDyP
contact email	virginie.brun@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD010396
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD010396

PRIDE project URI

Repository Record List

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