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PXD010396-1

PXD010396 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUbi complex characterization .
DescriptionUbiquinone (UQ) is a polyprenylated lipid that is conserved from bacteria to humans and is crucial to cellular respiration. How the cell orchestrates the efficient synthesis of UQ, which involves the modification of extremely hydrophobic substrates by multiple sequential enzymes, remains an unresolved issue. Here, we demonstrate that seven Ubi proteins form the Ubi complex, a stable metabolon that catalyzes the last six reactions of the UQ biosynthetic pathway in Escherichia coli. In addition to five Ubi enzymes, the 1 MDa Ubi complex contains the newly identified UbiJ and UbiK proteins that we show to be involved in the binding of UQ intermediates and the structuration of the multiprotein complex. We also develop an in vitro assay and show that UQ biosynthesis occurs in the cytoplasmic fraction of E. coli, from which we purify the Ubi complex. Collectively, our work documents a rare case of stable metabolon and revisits the current thinking that the hydrophobic intermediates of the UQ pathway are metabolized in association with the membrane.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_10:15:58.469.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD010396
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterYohann Couté
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-12 02:26:25ID requested
12024-10-07 10:15:59announced
Publication List
10.6019/PXD010396;
10.1016/j.chembiol.2018.12.001;
Hajj Chehade M, Pelosi L, Fyfe CD, Loiseau L, Rascalou B, Brugi, è, re S, Kazemzadeh K, Vo CD, Ciccone L, Aussel L, Cout, é Y, Fontecave M, Barras F, Lombard M, Pierrel F, A Soluble Metabolon Synthesizes the Isoprenoid Lipid Ubiquinone. Cell Chem Biol, 26(4):482-492.e7(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: ubiquinone, multiprotein complex,E. coli
Contact List
Virginie Brun
contact affiliationEDyP
contact emailvirginie.brun@cea.fr
lab head
Yohann Couté
contact affiliationEDyP
contact emailyohann.coute@cea.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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