PXD010361-2

PXD010361 is an original dataset announced via ProteomeXchange.

Title	Cell Surface Proteome during Enterohaemorrhagic E. coli infection
Description	Proteins on the cell surface are crucial for the interaction of a cell with its surrounding environment. Viral infection is known to remodel to the host cell surface to the benefit of the pathogen, but relatively little is known about how bacterial pathogens alter the host cell surface. Enterohaemorrhagic E. coli(EHEC) infects the apical surface of gut epithelium, and maniplates multiple aspects of host physiology. Here we used quantitative cell surface proteomics to investigate EHEC-induced changes to the host cell surface and show that the complement regulatory protein CD55 is cleaved from epithelial surfaces by the EHEC metalloprotease StcE. As a consequence of this, neutrophil attachment to the apical surface of epithelial cells is increased. This study is the first to apply quantitaive cell surface proteomics to EHEC infection and reveals a novel mechanism by which EHEC manipulates the host immune system.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:46:42.366.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Funriss
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-10 05:19:40	ID requested
1	2018-09-10 00:05:49	announced
⏵ 2	2024-10-22 04:46:50	announced	2024-10-22: Updated project metadata.

10.1074/jbc.ra118.005114;

Furniss RCD, Low WW, Mavridou DAI, Dagley LF, Webb AI, Tate EW, Clements A, infection reveals that the metalloprotease StcE cleaves CD55 from host epithelial surfaces. J Biol Chem, 293(44):17188-17199(2018) [pubmed]

submitter keyword: EHEC, Human, HeLa, LC-MS/MS

Abigail Clements
contact affiliation	MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Imperial College London, London SW7 2AZ
contact email	a.clements@imperial.ac.uk
lab head
Christopher Funriss
contact affiliation	Imperial College London
contact email	r.furniss12@imperial.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD010361

PRIDE project URI

• PRIDE
  1. PXD010361
     1. Label: PRIDE project
     2. Name: Cell Surface Proteome during Enterohaemorrhagic E. coli infection