PXD010359-1

PXD010359 is an original dataset announced via ProteomeXchange.

Title	CotL, a new morphogenetic coat protein of Clostridium difficile
Description	The strict anaerobe Clostridium difficile is an important nosocomial enteropathogen that has become the most common cause of antibiotic-associated diarrhea. The oxygen-resistant C. difficile spores play a central role in the infectious cycle, contributing to transmission, infection and recurrence. The spore surface layers, coat and exosporium, enable resistance of the spores to extreme physical and chemical stresses. Despite the critical importance of the spore in C. difficile infection, little is known about the mechanisms that orchestrate the assembly of its external layers. In this study, we identified and characterized a new C. difficile spore protein, named CotL, which is required for the assembly of the spore surface layers. The cotL gene was expressed in the mother cell compartment under the dual control of σE and σK. The CotL protein was localized in the C. difficile spore coat and cotL mutant spores had a major morphologic defect at the level of the coat/exosporium layers, as observed by transmission electron microscopy. Accordingly, spores of the cotL mutant contained a reduced amount of several proteins of the coat and exosporium, including CotB, CotE and CdeC. Additionally, cotL inactivation resulted in a defect in localization of late spore coat proteins in sporulating cells. Finally, spores of the cotL mutant were more sensitive to lysozyme and were impaired in germination. These defects are most likely associated with the structurally altered coat. Collectively, these results strongly suggest that CotL is a morphogenetic protein essential for the assembly of the spore coat in C. difficile.
HostingRepository	PRIDE
AnnounceDate	2019-10-16
AnnouncementXML	Submission_2019-10-16_04:54:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Douché
SpeciesList	scientific name: Peptoclostridium difficile (strain 630) (Clostridium difficile); NCBI TaxID: 272563;
ModificationList	L-methionine sulfoxide; N-acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-10 05:14:53	ID requested
⏵ 1	2019-10-16 04:54:32	announced
2	2024-10-22 04:46:29	announced	2024-10-22: Updated project metadata.

Alves Feliciano C, Douch, é T, Giai Gianetto Q, Matondo M, Martin-Verstraete I, Dupuy B, CotL, a new morphogenetic spore coat protein of Clostridium difficile. Environ Microbiol, 21(3):984-1003(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Clostridium difficile, spore coat, shotgun proteomics, Q Exactive HF

Bruno Dupuy
contact affiliation	Laboratoire Pathogenèse des Bactéries Anaérobies, InstitutPasteur, Paris, France
contact email	bruno.dupuy@pasteur.fr
lab head
Thibaut Douché
contact affiliation	Institut Pasteur
contact email	thibaut.douche@pasteur.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010359
     1. Label: PRIDE project
     2. Name: CotL, a new morphogenetic coat protein of Clostridium difficile