PXD010338-2

PXD010338 is an original dataset announced via ProteomeXchange.

Title	The garlic compound allicin causes strong disulfide stress and protein S-sulfoallylation as revealed by proteomics and transcriptomics in Staphylococcus aureus
Description	Allicin from garlic is an antimicrobial substance that is effective against several ESKAPE pathogens, including methicillin-resistant Staphylococcus aureus (MRSA). However, the antimicrobial mode action of allicin has not been revealed in Gram-positive bacteria. Here, we have studied the changes in the transcriptome by Allicin which revealed a thiol-specific oxidative stress response in S. aureus. Using shotgun proteomics, we identified numerous proteins that were modified by protein S-sulfoallylation in S. aureus. Allicin further caused an oxidative shift in the bacillithiol (BSH) redox potential as revealed by Brx-roGFP2 biosensor measurements. In summary, our results revealed that allicin acts via sulfoallylation of Cys residues in proteins causing an impaired redox state in S. aureus.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:37.368.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tobias Busche
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	L-methionine sulfoxide; N-ethylmaleimide derivatized cysteine
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-09 01:24:49	ID requested
1	2022-02-28 02:54:34	announced
⏵ 2	2023-11-14 08:49:37	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: proteomics, allicin stress, transcriptomics,Staphylococcus aureus, S-sulfoallylation

Haike Antelmann
contact affiliation	Freie Universität Berlin, Institute for Biology-Microbiology, Königin-Luise-Strasse 12-16, D-14195 Berlin, Germany.
contact email	haike.antelmann@fu-berlin.de
lab head
Tobias Busche
contact affiliation	Freie Universität Berlin, Institute for Biology-Microbiology, D-14195 Berlin, Germany
contact email	tbusche@cebitec.uni-bielefeld.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010338
     1. Label: PRIDE project
     2. Name: The garlic compound allicin causes strong disulfide stress and protein S-sulfoallylation as revealed by proteomics and transcriptomics in Staphylococcus aureus