PXD010333-2

PXD010333 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Analysis of intact mannose-6-phosphate glycopeptides
Description	We have repurposed standard phosphoproteomics workflow based on Fe3+IMAC for enrichment ofmannose-6-phosphate modified glycopeptides. This worklfow was used to profile lysosomal acid hydrolases in HeLa and CHO cell lines. We have combined this approach with CRISPR/Cas9 KO of acid phosphatases 2 and 5 responsible for dephosphorylation of mannose-6-phosphate glycopeptides in the lysosome. This combined approach enabled significantly deeper coverage of CHO mannose-6-phosphate glycoproteome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:46:15.475.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010333
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	TOMISLAV CAVAL
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-06 05:56:32	ID requested
1	2018-12-18 10:43:48	announced
⏵ 2	2024-10-22 04:46:16	announced	2024-10-22: Updated project metadata.

Publication List

10.6019/PXD010333;
10.1074/mcp.ra118.000967;
Č, aval T, Zhu J, Tian W, Remmelzwaal S, Yang Z, Clausen H, Heck AJR, Targeted Analysis of Lysosomal Directed Proteins and Their Sites of Mannose-6-phosphate Modification. Mol Cell Proteomics, 18(1):16-27(2019) [pubmed]

10.6019/PXD010333;

10.1074/mcp.ra118.000967;

Č, aval T, Zhu J, Tian W, Remmelzwaal S, Yang Z, Clausen H, Heck AJR, Targeted Analysis of Lysosomal Directed Proteins and Their Sites of Mannose-6-phosphate Modification. Mol Cell Proteomics, 18(1):16-27(2019) [pubmed]

Keyword List

curator keyword: Biological
submitter keyword: IMAC, Hela, M6P, glycopeptide,CHO

curator keyword: Biological

submitter keyword: IMAC, Hela, M6P, glycopeptide,CHO

Contact List

Albert J R Heck
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Science4Life, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands
contact email	A.J.R.Heck@uu.nl
lab head
TOMISLAV CAVAL
contact affiliation	Utrecht University
contact email	t.caval@uu.nl
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD010333
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD010333

PRIDE project URI

Repository Record List

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