PXD010315 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Improving fractionation of human milk proteins through calcium phosphate co-precipitation and their rapid characterization by capillary electrophoresis |
Description | This work describes a simple sample pretreatment method for the fractionation of human milk proteins (into their two main groups: whey and caseins) prior to their analysis. The protein extraction protocol is based on the addition of calcium phosphate to non-adjusted pH human milk. The combination of calcium ions with phosphate results in an effective co-precipitation of caseins. To assess the suitability of this fractionation protocol, the protein extracts were analyzed by SDS-PAGE, LC-MS/MS and CE analysis. The results evidence a significant decrease in contamination of casein fraction with whey proteins and vice versa compared with the conventional isoelectric precipitation of caseins. In addition, CE fraction collection coupled to LC-MS/MS (off-line coupling) has been successfully applied to the identification of minor proteins in this complex matrix. The methodology presented here constitutes a promising tool to enlarge the knowledge of human milk proteome. |
HostingRepository | PRIDE |
AnnounceDate | 2018-08-31 |
AnnouncementXML | Submission_2018-08-31_06:33:24.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD010315 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Luz Valero |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Deamidated; Oxidation; Carbamidomethyl |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-07-05 02:31:31 | ID requested | |
⏵ 1 | 2018-08-31 06:33:25 | announced | |
Publication List
Ten-Dom, é, nech I, Sim, ó, -Alfonso EF, Herrero-Mart, í, nez JM, Improving Fractionation of Human Milk Proteins through Calcium Phosphate Coprecipitation and Their Rapid Characterization by Capillary Electrophoresis. J Proteome Res, 17(10):3557-3564(2018) [pubmed] |
Keyword List
curator keyword: Technical |
submitter keyword: calcium phosphate fractionation |
human milk whey proteins |
human milk caseins |
capillary electrophoresis |
fraction collection |
Contact List
Dr. José Manuel Herrero-Martínez |
contact affiliation | Department of Analytical Chemistry, University of ValenciCC. Doctor Moliner 50, E-46100 Burjassot, Valencia, Spain |
contact email | jmherrer@uv.es |
lab head | |
Luz Valero |
contact affiliation | Universitat de Valencia |
contact email | mluz.valero@uv.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010315
- Label: PRIDE project
- Name: Improving fractionation of human milk proteins through calcium phosphate co-precipitation and their rapid characterization by capillary electrophoresis