PXD010313-2

PXD010313 is an original dataset announced via ProteomeXchange.

Title	Oryza sativa AM fungi interaction
Description	We describe a method that detects membrane proteins from cell type-specific plant plasma membrane subdomains. As the biochemical properties of membrane subdomains are often unknown, general membrane enrichment by continuous sucrose density gradient centrifugation was combined with filter-aided sample preparation, strong cation exchange pre-fractionation and sensitive LTQ Orbitrap Velos-based mass-spectrometry proteomics. This workflow identified plasma membrane proteins, including underrepresented proteins, at an unprecedented level of sensitivity and reproducibility
HostingRepository	PRIDE
AnnounceDate	2018-09-24
AnnouncementXML	Submission_2018-11-13_06:01:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marco Chiapello
SpeciesList	scientific name: Oryza sativa subsp. japonica (Rice); NCBI TaxID: 39947;
ModificationList	2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-05 01:41:43	ID requested
1	2018-09-24 07:01:49	announced
⏵ 2	2018-11-13 06:01:39	announced	Updated publication reference for PubMed record(s): 30410018.
3	2024-10-22 04:46:26	announced	2024-10-22: Updated project metadata.

Roth R, Chiapello M, Montero H, Gehrig P, Grossmann J, O'Holleran K, Hartken D, Walters F, Yang SY, Hillmer S, Schumacher K, Bowden S, Craze M, Wallington EJ, Miyao A, Sawers R, Martinoia E, Paszkowski U, A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane. Nat Commun, 9(1):4677(2018) [pubmed]

curator keyword: Biological

submitter keyword: Oryza sativa, proteomics, periarbuscular membrane, FASP, membrane enrichment

Uta Paszkowski
contact affiliation	Plant Science, University of Cambridge, UK
contact email	up220@cam.ac.uk
lab head
Marco Chiapello
contact affiliation	University of Cambridge, UK
contact email	chiapello.m@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD010313

PRIDE project URI

• PRIDE
  1. PXD010313
     1. Label: PRIDE project
     2. Name: Oryza sativa AM fungi interaction