PXD010304-1

PXD010304 is an original dataset announced via ProteomeXchange.

Title	Proteome and secretome of HepaRG cells
Description	Primary human hepatocytes, the gold standard for in vitro studies of liver-related functions, suffer from uncertain availability and high variability in cell activity. Over years, a number of alternative hepatic cell lines have lost major liver-like functions, but not HepaRG cells. Therefore, their increasing use worldwide today arouses the need for establishing a reference functional status of differentiated HepaRG cells known as HPR116, which originate from the initial cell bank. Deep proteome and secretome analyses enabled us to show that they nicely express, at levels generally close to those in primary hepatocytes, master regulators of the hepatic phenotype, structural elements that ensure liver-like polarisation and factors supporting their transdifferentiation properties. Their highly differentiated status, mitochondrial functionality, hepatokine secretion ability and response to insulin was verified. Overall, the HepaRG cell system appears as robust surrogate cell system to primary hepatocytes, versatile enough to study not only xenobiotic detoxification but also the control of hepatic energy metabolism, secretory function and disease-related signalling pathways.
HostingRepository	PRIDE
AnnounceDate	2019-02-27
AnnouncementXML	Submission_2019-02-27_00:49:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabrice BERTILE
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	impact HD; amaZon ETD

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-04 08:36:54	ID requested
⏵ 1	2019-02-27 00:49:54	announced

Tascher G, Burban A, Camus S, Plumel M, Chanon S, Le Guevel R, Shevchenko V, Van Dorsselaer A, Lefai E, Guguen-Guillouzo C, Bertile F, In-Depth Proteome Analysis Highlights HepaRG Cells as a Versatile Cell System Surrogate for Primary Human Hepatocytes. Cells, 8(2):(2019) [pubmed]

curator keyword: Biological

submitter keyword: HPR116, proteome, secretome, functional assays, copy numbers, surrogate cell system to PHH

Sarah Cianférani
contact affiliation	Laboratoire de Spectrométrie de Masse BioOrganique, Analytical Science department, IPHC, CNRS, Strasbourg University, UMR7178, Strasbourg, France
contact email	sarah.cianferani@unistra.fr
lab head
Fabrice BERTILE
contact affiliation	CNRS
contact email	fbertile@unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD010304

PRIDE project URI

• PRIDE
  1. PXD010304
     1. Label: PRIDE project
     2. Name: Proteome and secretome of HepaRG cells