PXD010285 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of O-GlcNAcylated proteins in the protozoan parasite Trypanosoma cruzi |
| Description | Trypanosoma cruzi is the causal agent of Chagas’ disease, which is one of the biggest public health problems in Latin America. Several post-translational modifications (PTM) have been studied in T. cruzi but there is no work focused on the O-GlcNAcylation, which is a highly conserved monosaccharide PTM found on serine and threonine residues of proteins mainly from the nucleus, cytoplasm and mitochondria. It is thought to regulate proteins in a manner analogous to protein phosphorylation, even their crosstalk allows the regulation of cellular functions in response to nutrients and stress. In the present work we demonstrate O-GlcNacylation in T. cruzi epimastigotes using two different methods: western blot with specific antibodies and click chemistry labelling. Then, we identify 2033 O-GlcNAcilated proteins and 48 modification sequences by mass spectrometry. Most of this proteins are part of structures, functions and pathways that are essentials for the parasite survival and evolution. For the first time O-GlcNacylation is idenytified in T. cruzi proteins and due to the many biological processes in which they participate, this study opens a new research field in Trypanosomatids biology, infection processes and potential identification of therapeutic targets. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_04:46:24.593.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Luc Camoin |
| SpeciesList | scientific name: Trypanosoma cruzi strain CL Brener; NCBI TaxID: 353153; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2018-07-02 07:58:02 | ID requested | |
| 1 | 2019-04-18 02:19:56 | announced | |
| ⏵ 2 | 2024-10-22 04:46:30 | announced | 2024-10-22: Updated project metadata. |
Publication List
| Torres-Guti, é, rrez E, P, é, rez-Cervera Y, Camoin L, Zenteno E, Aquino-Gil MO, Lefebvre T, Cabrera-Bravo M, Reynoso-Ducoing O, Bucio-Torres MI, Salazar-Schettino PM, . Front Endocrinol (Lausanne), 10():199(2019) [pubmed] |
| 10.3389/fendo.2019.00199; |
Keyword List
| curator keyword: Biological |
| submitter keyword: PTM, O-GlcNAc, click chemistry,Trypanosoma cruzi, epimastigote |
Contact List
| Luc Camoin |
|---|
| contact affiliation | Aix-Marseille univ, CNRS, Inserm, Institut Paoli-Calmettes, CRCM, Marseille Protéomique |
| contact email | luc.camoin@inser.fr |
| lab head | |
| Luc Camoin |
|---|
| contact affiliation | Life Sciences |
| contact email | luc.camoin@inserm.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD010285 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010285
- Label: PRIDE project
- Name: Identification of O-GlcNAcylated proteins in the protozoan parasite Trypanosoma cruzi
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