PXD010236-1

PXD010236 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Time-Course Mass Spectrometry Data of Adipose Mesenchymal Stem Cells Acquiring Chondrogenic Phenotype
Description	Project description: Adipose mesenchymal stem cells (ADSCs) are fast gaining popularity in regenerative medicine for their pluripotency and relative use of collection. Currently, we are addressing the use of ADSCs in otolaryngology, for the treatment of vocal fold (VF) paralysis. VF can result from accidentally sectioning the recurrent laryngeal nerve (RLN) during thyroidectomy. This prevents closure of the upper larynx and promotes food aspiration into the lungs. Volume augmenting materials are sometimes injected in order to bring the paralyzed VF in close proximity to the counter lateral VF. The procedure is called injection medialization laryngoplasty (IML); we have recently developed a rabbit IML model for testing biological reagents in order to improve outcomes. As part of these studies ADSCs were differentiated into chondrocytes and their fate was studied in vitro and in situ. We used mass spectrometry proteomics because microarrays based gene expression platforms do not report actual protein levels. The data show protein levels in controls and chondrogenically differentiating rabbit ADSCs at 0, 14 and 28 days, analyzed using nano-flow liquid chromatography electrospray ionization tandem mass spectrometry (nLC-MS/MS).
HostingRepository	MassIVE
AnnounceDate	2018-07-12
AnnouncementXML	Submission_2018-07-12_10:02:22.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Serban San-MArina
SpeciesList	scientific name: Oryctolagus cuniculus; common name: rabbit; NCBI TaxID: 9986;
ModificationList	unknown modification: Carbamidomethylation of cysteine and oxidation of methionine were allowed as variable modifications
Instrument	instrument model: Thermo Scientific Q-Exactive Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany) coupled to a Thermo Ultimate 3000 RSLC nano HPLC system

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-26 13:06:44	ID requested
⏵ 1	2018-07-12 10:02:22	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Proteomics, mass spectrometry, adipose mesenchymal stem cells, regenerative medicine, chondrogenic time-course

submitter keyword: Proteomics, mass spectrometry, adipose mesenchymal stem cells, regenerative medicine, chondrogenic time-course

Contact List

Serban San-Marina
contact affiliation	Mayo, Rochester, MN
contact email	sanmarina.serban@mayo.edu
lab head
Serban San-MArina
contact affiliation	Mayo Clinic Rochester
contact email	sanmarina.serban@mayo.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082505