PXD010201-1

PXD010201 is an original dataset announced via ProteomeXchange.

Title	The human allicin-proteome: global protein S-thioallylation of by the garlic defence substance allicin and its biological effects
Description	A single clove of edible garlic (Allium sativum L.) produces up to 5 mg of allicin (diallylthiosulfinate), a thiol-reactive sulfur-containing defence substance that gives injured garlic tissue its characteristic smell. Allicin induces apoptosis or necrosis in a dose-dependent manner but sublethal doses influence cellular metabolism and signalling cascades. Oxidation of protein thiols and depletion of the glutathione pool are thought to be responsible for allicin’s physiological effects. Here, we studied the effect of Allicin on post-translational thiol-modification in human Jurkat cells using shotgun LC-MS/MS analyses. We identified 332 proteins in the human Jurkat cell proteome that were modified by S-thioallylation which causes a mass shift of 72 Dalton on cysteines. Many S-thioallylated proteins are high abundant proteins, including the cytoskeletal proteins tubulin, actin, cofilin, filamin and plastin-2, the heat shock chaperones HSP90 and HSPA4, the glycolytic enzymes GAPDH, ALDOA, PKM as well the protein translation factor EEF2. We confirmed that Allicin disrupted the actin cytoskeleton in mouseL929 fibroblasts. Allicin further resulted in Zn2+ release from proteins and stimulated the Zn2+-dependent IL1-triggered release of IL2 in murine EL4 T-cells. Allicin further caused inhibition of enolase activity. In conclusion, our study revealed the overall extent of widespread S-thioallylation in the human Jurkat cell proteome after allicin exposure which affects essential cellular functions of selected allicin targets.
HostingRepository	PRIDE
AnnounceDate	2018-11-30
AnnouncementXML	Submission_2018-11-30_08:02:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Van Loi Vu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-methionine sulfoxide; N-ethylmaleimide derivatized cysteine
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-22 08:09:53	ID requested
⏵ 1	2018-11-30 08:02:59	announced
2	2018-12-04 04:54:24	announced	Updated publication reference for PubMed record(s): 30500420.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Allicin, S-thioallylation, protein modification, cysteine, cytoskeleton, actin, enolase, zinc, Jurkat, fibroblasts, T-cells, interleukin IL1, IL2

Prof. Dr. Haike Antelmann
contact affiliation	Freie Universität Berlin Institut für Biologie-Mikrobiologie
contact email	haike.antelmann@fu-berlin.de
lab head
Van Loi Vu
contact affiliation	Freie Universität Berlin
contact email	vu.v.loi@fu-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010201
     1. Label: PRIDE project
     2. Name: The human allicin-proteome: global protein S-thioallylation of by the garlic defence substance allicin and its biological effects