PXD010168-3

PXD010168 is an original dataset announced via ProteomeXchange.

Title	Sperm proteome of apyrene and eupyrene sperm in Carolina Sphinx moth Manduca sexta, and monarch butterfly Danaus plexippus
Description	All but the most basal Lepidopteran species produce two sperm morphs. Only one of these morphs is capable of completing karyogamy and producing offspring, this morph contains the correct genetic complement and is termed eupyrene sperm. Apyrene sperm, on the other hand, is completely devoid of nuclear DNA and fertilisation incompetent. Despite the fact apyrene sperm is believed to be functional, the function of this sperm type is largely unknown. Here we apply tandem mass spectrometry based proteomics to the two sperm types independently in the monarch butterfly (Danaus plexippus) and the carolina sphinx moth (Manduca sexta). Comparative analysis between sperm morphs and species shows a reduced complexity and greater divergence in apyrene sperm relative to eupyrene consistent across the two species.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:57.774.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emma Whittington
SpeciesList	scientific name: Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm); NCBI TaxID: 7130; scientific name: Danaus plexippus; NCBI TaxID: 13037;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-19 08:07:05	ID requested
1	2019-05-06 07:13:41	announced
2	2019-07-12 04:30:28	announced	Updated publication reference for PubMed record(s): 31268533.
⏵ 3	2024-10-22 04:46:02	announced	2024-10-22: Updated project metadata.

10.1093/gbe/evz080;

Whittington E, Karr TL, Mongue AJ, Dorus S, Walters JR, Evolutionary Proteomics Reveals Distinct Patterns of Complexity and Divergence between Lepidopteran Sperm Morphs. Genome Biol Evol, 11(7):1838-1846(2019) [pubmed]

curator keyword: Biological

submitter keyword: monarch, manduca sexta, sperm heteromorphism,Lepidoptera, sperm

Dr Steve Dorus
contact affiliation	Syracuse University
contact email	sdorus@syr.edu
lab head
Emma Whittington
contact affiliation	Syracuse Univeristy
contact email	ewhittin@syr.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD010168

PRIDE project URI

• PRIDE
  1. PXD010168
     1. Label: PRIDE project
     2. Name: Sperm proteome of apyrene and eupyrene sperm in Carolina Sphinx moth Manduca sexta, and monarch butterfly Danaus plexippus