PXD010093-2

PXD010093 is an original dataset announced via ProteomeXchange.

Title	2018CHPP - Digging for Missing Proteins Using Low-Molecular-Weight Protein Enrichment and a "Mirror Protease" Strategy
Description	ABSTRACT Since 2012, Chromosome-Centric Human Proteome Project (C-HPP) launched the journey of missing proteins (MPs) investigation for completing human proteome project (HPP). We found the majority of MPs were distributed in low molecular weight (LMW) ranges, especially in 0-40 kDa. The identification of LMW proteins is challenging owing to their low length, low abundancy, and hydrophobicity. Even worse, many sequences from Trypsin digestion are unlikely to yield detectable peptides or composed less number of observable peptides in MS detection. Therefore, we focused on small MPs by combining LMW protein enrichment and mirror-protease strategy on human testis samples. The in-depth testis LMW protein profiling documented 4,063 proteins from which 2,565 proteins were belong to LMW proteins and 1,130 proteins had mirror peptides. This provided mass spectral evidence for small MPs further verification, and two MPs were verified in seven identified MPs. For Q8N688, confident peptides were verified from Trypsin and LysargiNase digestions, and continuous b/y-ion peptide was obtained by mirror-spectrum matching. Two verified peptides of Q86WR6 were from gel-separation and gel-elution dataset, respectively. And the other 5 MPs cannot be distinguished sufficiently as PE1, but need more verification information.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:43.340.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010093
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	cuitong he
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-11 07:23:05	ID requested
1	2018-10-04 09:34:00	announced
⏵ 2	2024-10-22 04:45:51	announced	2024-10-22: Updated project metadata.

He C, Sun J, Shi J, Wang Y, Zhao J, Wu S, Chang L, Gao H, Liu F, Lv Z, He F, Zhang Y, Xu P, Digging for Missing Proteins Using Low-Molecular-Weight Protein Enrichment and a "Mirror Protease" Strategy. J Proteome Res, 17(12):4178-4185(2018) [pubmed]

10.6019/PXD010093;

10.1021/acs.jproteome.8b00398;

ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), China Human Proteome Project (CNHPP), Human Proteome Project

submitter keyword: testis, low molecular weight,Chromosome-Centric Human Proteome Project, mirror-protease, missing proteins

Ping Xu
contact affiliation	Ping Xu, Ph.D. Secretary General of CNHUPO Professor, Director Department of Genomics and Proteomics, State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing) Beijing Institute of Lifeomics， 38 Life Science Park Road, Beijing 102206, P.R. China. Tel: 0086-10-61777113, Fax: 0086-10-61777060
contact email	xuping_bprc@126.com
lab head
cuitong he
contact affiliation	National Center for Protein Sciences·Beijing
contact email	hecuitongpro@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD010093

PRIDE project URI

• PRIDE
  1. PXD010093
     1. Label: PRIDE project
     2. Name: 2018CHPP - Digging for Missing Proteins Using Low-Molecular-Weight Protein Enrichment and a "Mirror Protease" Strategy