PXD010088 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Transformation-induced stress at telomeres is counteracted through changes in the telomeric proteome including SAMHD1 |
Description | Telomeres play crucial roles during tumorigenesis inducing cellular senescence upon telomere shortening and extensive chromosome instability during telomere crisis. However, it has not been investigated if and how cellular transformation and oncogenic stress alters telomeric chromatin composition and function. Here we transform human fibroblasts by consecutive transduction with vectors expressing hTERT, the SV40 early region and activated H-RasV12. Pairwise comparisons of the telomeric proteome during different stages of transformation reveals upregulation of proteins involved in chromatin remodeling, DNA repair and replication at chromosome ends. Depletion of several of these proteins induces telomere fragility indicating their roles in replication of telomeric DNA. Depletion of SAMHD1, which has reported roles in DNA resection and homology directed repair, leads to telomere breakage events in cells deprived of the shelterin component TRF1. Thus our analysis identifies factors, which accumulate at telomeres during cellular transformation to promote telomere replication and repair, resisting oncogene-borne telomere replication stress. |
HostingRepository | PRIDE |
AnnounceDate | 2018-07-13 |
AnnouncementXML | Submission_2018-07-13_08:14:13.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Romain Hamelin |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-06-11 04:44:24 | ID requested | |
⏵ 1 | 2018-07-13 08:14:14 | announced | |
2 | 2018-11-22 01:11:36 | announced | Updated publication reference for PubMed record(s): 30456372. |
3 | 2024-10-22 04:45:40 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Quantitative proteomics, replication stress, SAMHD1, telomeres, transformation |
Contact List
Joachim Lingner |
contact affiliation | Ecole Polytechnique Fédérale de Lausanne (EPFL), School of Life Sciences, 1015 Lausanne, Switzerland. Swiss Institute for Experimental Cancer Research (ISREC) at EPFL, Station 19, CH-1015 Lausanne, Switzerland |
contact email | joachim.lingner@epfl.ch |
lab head | |
Romain Hamelin |
contact affiliation | PCF |
contact email | romain.hamelin@epfl.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010088
- Label: PRIDE project
- Name: Transformation-induced stress at telomeres is counteracted through changes in the telomeric proteome including SAMHD1