PXD010083-2

PXD010083 is an original dataset announced via ProteomeXchange.

Title	Characterization of loquat (Eriobotrya japonica Lindl.) honey botanical origin according to its chitinase activity
Description	Bees make honey from the nectar that they collect from flowers. The characteristics of honey are closely associated to original botanical species. Compare with sugars in honey, proteins are minor components but usually used as an important honey quality evaluation parameters. Flower-origin proteins could be a good marker for the authentication. However, as a minute component in honey proteome, plant origin proteins are hard to be detected in honey by regular proteomic approaches, such as gel-based techniques. In this study, Eriobotrya japonica Lindl. (loquat) nectar and its derivative monofloral honey were systematically compared, especially regarding the proteomes and enzymatic activities. Using two-dimensional electrophoresis and mass spectrometry, only bee-originated proteins were detected in loquat honey which were major royal jelly proteins and two uncharacterized proteins. Xylosidase, thaumatin, and two kinds of chitinases were detected in loquat floral nectar by the gel-based proteomic approach. To our knowledge, it is the first study to analysis nectar-originated enzymes’ activity in honey and we proposed that the zymography of chitinase is a potential marker for honey botanical origin authentication.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:44.321.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hong-Guang Zha
SpeciesList	scientific name: Eriobotrya japonica; NCBI TaxID: 32224; scientific name: Apis mellifera mellifera; NCBI TaxID: 44477;
ModificationList	No PTMs are included in the dataset
Instrument	5800 TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-11 01:35:05	ID requested
1	2019-02-15 06:46:11	announced
⏵ 2	2024-10-22 04:45:45	announced	2024-10-22: Updated project metadata.

Song YQ, Milne RI, Zhou HX, Ma XL, Fang JY, Zha HG, Floral nectar chitinase is a potential marker for monofloral honey botanical origin authentication: A case study from loquat (Eriobotrya japonica Lindl.). Food Chem, 282():76-83(2019) [pubmed]

10.1016/j.foodchem.2018.12.107;

submitter keyword: Authentication

Botanical origin；Chitinase

Eriobotrya japonica Lindl.

Floral nectar

Monofloral honey

Nectarin

Hong-Guang Zha
contact affiliation	Huangshan University
contact email	arctos725@outlook.com
lab head
Hong-Guang Zha
contact affiliation	Huangshan University
contact email	arctos725@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010083
     1. Label: PRIDE project
     2. Name: Characterization of loquat (Eriobotrya japonica Lindl.) honey botanical origin according to its chitinase activity