PXD010067-1

PXD010067 is an original dataset announced via ProteomeXchange.

Title	Characterization of a L-Gulono-1,4-Lactone Oxidase Like Protein in the Floral Nectar of Mucuna sempervirens, Fabaceae.
Description	Floral nectar plays important roles in the interaction between animal-pollinated plants and pollinators. Growing empirical evidence shows that most of the proteins secreted in nectar (nectarins) are enzymes that can tailor nectar chemistry for their animal mutualists or reduce the growth of microorganisms in nectar. However, to date, the function of many nectarins remains unknown, and very few plant species have had their nectar proteome thoroughly investigated. Mucuna sempervirens (Fabaceae) is a perennial woody vine native to China. Nectarins from this species were separated using two-dimensional gel electrophoresis, and analyzed using mass spectrometry. A L-gulonolactone oxidase like protein (MsGulLO) was detected. MsGulLO has high similarity to L-gulonolactone oxidase 5 (AtGulLO5) in Arabidopsis thaliana, which was suggested to be involved in the pathway of ascorbate biosynthesis; however both MsGulLO and AtGulLO5 are divergent from animal L-gulonolactone oxidases. MsGulLO is suggested to function in hydrogen peroxide generation in nectar but not in plant ascorbate biosynthesis.
HostingRepository	PRIDE
AnnounceDate	2018-08-16
AnnouncementXML	Submission_2018-08-16_07:44:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hong-Guang Zha
SpeciesList	scientific name: Mucuna sempervirens; NCBI TaxID: 690556;
ModificationList	iodoacetamide derivatized residue
Instrument	TOF/TOF 5800

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-08 01:33:36	ID requested
⏵ 1	2018-08-16 07:44:24	announced

Zhou HX, Milne RI, Ma XL, Song YQ, Fang JY, Sun H, Zha HG, , Fabaceae. Front Plant Sci, 9():1109(2018) [pubmed]

curator keyword: Biological

submitter keyword: L-ascorbate biosynthesis

L-gulonolactone oxidase like protein

Mucuna sempervirens Hemsl

Nectarin

Hong-Guang Zha
contact affiliation	Huangshan University
contact email	arctos725@outlook.com
lab head
Hong-Guang Zha
contact affiliation	Huangshan University
contact email	arctos725@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010067
     1. Label: PRIDE project
     2. Name: Characterization of a L-Gulono-1,4-Lactone Oxidase Like Protein in the Floral Nectar of Mucuna sempervirens, Fabaceae.