PXD010060-1

PXD010060 is an original dataset announced via ProteomeXchange.

Title	Metaproteomic analysis of glacial ecosystem
Description	Glaciers are populated by a large number of microorganisms including bacteria, archaea and microeukaryotes. From an ecological point of view, three ecosystems can be differentiated in glaciers: the supraglacial ecosystem, the subglacial ecosystem and the englacial ecosystem. Several factors such as solar radiation, nutrient availability and water content greatly determine the diversity and abundance of microbial populations, the type of metabolism and the biogeochemical cycles. Firstly, the supraglacial ecosystem, sunlit and oxygenated, is predominantly populated by autotrophic microorganisms. Secondly, the subglacial ecosystem contains a majority of chemoautrotophs that are fed on the mineral salts of the rocks and basal soil. Lastly, the englacial ecosystem is the less studied and the one that contains the smallest number of microorganisms. However, these unknown englacial microorganisms establish a true trophic chain and appear to have an active metabolism. In order to study their metabolic potentials, samples of englacial ice were taken from an Antarctic glacier. The cells were harvested and their proteins were extracted and analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI/TOF/TOF). Several proteins and enzymes were found that demonstrate the existence of cellular activity at subzero temperatures. In this way it is shown that the englacial microorganisms are not quiescent, but that they maintain an active metabolism and play an important role in the glacial microbial community.
HostingRepository	PRIDE
AnnounceDate	2019-08-29
AnnouncementXML	Submission_2019-08-29_06:29:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristina Cid
SpeciesList	scientific name: environmental samples; NCBI TaxID: 48479;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-07 06:23:31	ID requested
⏵ 1	2019-08-29 06:29:43	announced
2	2023-11-14 08:49:55	announced	2023-11-14: Updated project metadata.

10.1038/s41598-019-47994-9;

curator keyword: Metaproteomics

submitter keyword: MALDI-TOF MS, culturomics, metaproteomics, glacier

Cristina Cid
contact affiliation	Microbial Evolution Laboratory, Centro de Astrobiologia (CSIC-INTA), 28850 Torrejón de Ardoz, Madrid, Spain
contact email	cidsc@inta.es
lab head
Cristina Cid
contact affiliation	Centro de Astrobiologia (CSIC-INTA), 28850 Torrejón de Ardoz, Madrid, Spain
contact email	cidsc@inta.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD010060

PRIDE project URI

• PRIDE
  1. PXD010060
     1. Label: PRIDE project
     2. Name: Metaproteomic analysis of glacial ecosystem