PXD010053-2

PXD010053 is an original dataset announced via ProteomeXchange.

Title	Mapping Proteome-Wide Targets of Protein Kinases in Response to Stresses
Description	Protein kinases are major regulatory components in almost all cellular processes in eukaryotic cells. By adding phosphate groups, protein kinases regulate the activity, localization, protein-protein interactions and other profiles of their target proteins. It is known that protein kinases such as SNF1 related protein kinase 2 and 3 (SnRK2 and SnRK3), calcium dependent protein kinases (CDPKs), and mitogen activated protein kinases (MAPKs) are central components in plant response to environmental stresses such as drought, high salinity, cold and pathogen attack. Although the phosphorylation-dependent signaling plays critical roles in plant stress biology, only a few targets of these protein kinases have been identified. How these protein kinases regulate the downstream biological processes and mediate the stress responses are still largely unknown. In this study, we developed an isotope labeled protein kinase assay-based approach with enriched phosphoproteome as kinase-substrates pool to identify the putative substrates of 8 protein kinases that function in plant abiotic and biotic stress responses. As a result, we identified more than 5,081 putative targets of osmotic stress-activated SnRK2.4 and SnRK2.6, ABA activated protein kinases SnRK2.6 and Casein-Kinase Like 2 (CKL2), elicitor-activated protein kinase CDPK11 and MPK6, cold-activated protein kinase MPK6, H2O2-activated protein kinase OXI1 and MPK6, salt-induced protein kinase SOS1 and MPK6, as well as the low-potassium-activated protein kinase CIPK23. We also analyzed the conserved motif and functional ontology of these putative kinase targets. These results provide comprehensive information on the role of these protein kinases in the control of cellular activities and could be valuable resource for further study on the mechanism underlying plant response to environmental stresses.
HostingRepository	PRIDE
AnnounceDate	2020-01-16
AnnouncementXML	Submission_2020-02-17_03:19:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuan-Chih Hsu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-07 01:37:43	ID requested
1	2020-01-16 00:59:03	announced
⏵ 2	2020-02-17 03:20:01	announced	2020-02-17: Updated publication reference for PubMed record(s): 31992638.
3	2024-10-22 04:45:50	announced	2024-10-22: Updated project metadata.

Wang P, Hsu CC, Du Y, Zhu P, Zhao C, Fu X, Zhang C, Paez JS, Macho AP, Tao WA, Zhu JK, Mapping proteome-wide targets of protein kinases in plant stress responses. Proc Natl Acad Sci U S A, 117(6):3270-3280(2020) [pubmed]

curator keyword: Biological

submitter keyword: Plant kinase, Stress, Phosphoproteomics

jian-Kang Zhu
contact affiliation	Shanghai Center for Plant Stress Biology
contact email	jkzhu@sibs.ac.cn
lab head
Chuan-Chih Hsu
contact affiliation	Purdue University
contact email	without1102@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010053
     1. Label: PRIDE project
     2. Name: Mapping Proteome-Wide Targets of Protein Kinases in Response to Stresses