PXD010047 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tomosyn is upregulated by IgE and functions as a PKC-regulated fusion clamp in mast cell degranulation |
Description | The regulation of soluble N ethylmaleimide sensitive factor attachment receptor (SNARE) mediated membrane fusion by upstream signaling events is poorly understood. Here we show that the SNARE binding protein tomosyn-1 negatively regulates type I IgE Fc receptor (FcRI) induced degranulation of mast cells. After FcRI stimulation, tomosyn-1 (also STXBP5) was phosphorylated on serine and threonine residues and dissociated from the SNARE protein syntaxin 4 (STX4), followed by association with syntaxin 3 (STX3). Protein kinase A (PKA) and protein kinase C (PKC) prevented these activities. We identified PKC as the major kinase required for tomosyn-1 threonine phosphorylation and for the regulation of the interaction with STXs. Incubation with high IgE concentrations induced tomosyn-1 expression in cultured mast cells. In basophils from allergic patients with normal total IgE serum titers tomosyn-1 expression was lower than in patients with high IgE titers who expressed tomosyn-1 to the same extent as non-allergic subjects. Our findings identified tomosyn-1 as a negative regulator of mast cell degranulation that required PKC to switch its interaction with STX partners during fusion. The IgE-mediated upregulation of tomosyn-1 in allergic patients may represent a counter-regulatory mechanism to limit disease development. |
HostingRepository | PRIDE |
AnnounceDate | 2018-07-04 |
AnnouncementXML | Submission_2018-07-04_06:00:17.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thibaut LEGER |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue; monohydroxylated residue; acetylated residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-06-06 07:20:03 | ID requested | |
⏵ 1 | 2018-07-04 06:00:18 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: immunoprepicipitations, mouse, Q-Exactive plus, mast cells, IgE FC receptor, phosphorylations |
Contact List
Ulrich Blank |
contact affiliation | Dr. Ulrich Blank Department chair and team leader Mast cells and Basophils in Inflammation and Remodeling Inserm U 1149 & CNRS ERL8252 Université Paris-Diderot Faculté de Médecine Site X. Bichat 16, rue Henri Huchard F-75870 Paris Cedex 18 FRANCE |
contact email | ulrich.blank@inserm.fr |
lab head | |
Thibaut LEGER |
contact affiliation | Institut Jacques Monod |
contact email | thibaut.leger@ijm.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010047
- Label: PRIDE project
- Name: Tomosyn is upregulated by IgE and functions as a PKC-regulated fusion clamp in mast cell degranulation